Distribution of catalase and its modulation by 12-O-tetradecanoylphorbol-13-acetate in murine dermis and subpopulations of keratinocytes differing in their stages of differentiation.
Distribution of catalase and its modulation by 12-O-tetradecanoylphorbol-13-acetate in murine dermis and subpopulations of keratinocytes differing in their stages of differentiation.
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过氧化氢酶的分布及其在小鼠真皮和不同分化阶段的角质形成细胞亚群中 12-O-十四烷酰佛波醇-13-乙酸酯的调节。
DOI：
10.1093/carcin/9.7.1259
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发表时间：
1988
期刊：
影响因子：
4.7
通讯作者：
Cantu,AR
中科院分区：
文献类型：
--
作者：
ReinersJr,JJ;Hale,MA;Cantu,AR
用12-O-十四烷酰基佛波醇-13-乙酸酯（TPA）局部治疗雌性SENCAR小鼠，在启动子应用后6小时和18小时内，真皮和表皮过氧化氢酶特异性活性分别降低了38%和51%。皮肤过氧化氢酶活性恢复到对照水平在72小时内的治疗，而表皮过氧化氢酶活性仍然受到抑制。活性测量也在四个亚群的角质形成细胞制备Percoll梯度离心，不同的分化阶段。过氧化氢酶的活性随着角质形成细胞的成熟而增加，范围从基底细胞制备物的45-54 U /mg蛋白到颗粒-鳞状细胞制备物的252 U /mg蛋白。用TPA（2 μg）预处理表皮16 - 18 h，在Percoll梯度离心法制备的所有角质形成细胞亚群中，过氧化氢酶比活性均降低46 - 52%。类似地，每个细胞的过氧化氢酶单位与每个细胞提取的蛋白质的图表明TPA处理的表皮的基底细胞和棘细胞角质形成细胞中的过氧化氢酶活性降低55 - 60%。此外，整个表皮匀浆中的过氧化氢酶特异性活性（144 - 182单位/mg蛋白）与颗粒/鳞状角质形成细胞亚群的活性最相似。总的来说，这些研究表明：（i）TPA降低了整个表皮过氧化氢酶的H2 O2解毒能力;（ii）对未分级表皮制剂的活性测量可能不代表基底细胞角质形成细胞群体。
Topical treatment of female SENCAR mice with 12-O-tetradecanoylphorbol- 13-acetate (TPA) reduced both dermal and epidermal catalase-specific activities 38% and 51% within 6 h and 18 h of promoter application, respectively. Dermal catalase activity recovered to control levels within 72 h of treatment whereas epidermal catalase activity remained suppressed. Activity measurements were also made in four subpopulations of keratinocytes prepared by Percoll gradient centrifugation that differed in their stages of differentiation. Catalase-specific activity increased with keratinocyte maturity and ranged from 45–54 U /mg protein for basal cell preparations to 252 U /mg protein for granular-squamous cell preparations. Pretreatment of the epidermis for 16 –18 h with TPA (2 μg) uniformly reduced catalase-specific activity 46 –52 % in all keratinocyte subpopulations prepared by Percoll gradient centrifugation. Similarly, plots of catalase units per cell versus extracted protein per cell suggested 55 –60 % decreases in catalase activity in basal and spinous cell keratinocytes of TPA treated epidermis. Furthermore, catalase-specific activity in homogenates of whole epidermis (144 –182 units /mg protein) was most similar to the activity of the granular/squamous keratinocyte subpopulation. Collectively, these studies suggest that: (i) TPA reduces the capacity for H2O2detoxification by catalase throughout the epidermis; and (ii) activity measurements on unfractionated epidermal preparations may not be representative of the basal cell keratinocyte population.
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DOI：
10.1016/0005-2760(85)90023-2
发表时间：
1985-06
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
J. Turk;B. Wolf;P. Comens;J. Colca;B. Jakschik;M. Mcdaniel
通讯作者：
J. Turk;B. Wolf;P. Comens;J. Colca;B. Jakschik;M. Mcdaniel
(12R)-和(12S)-羟基二十碳四烯酸激活白细胞运动并从白细胞膜制剂中置换[3H]白三烯B4。
DOI：
10.1016/0005-2760(87)90119-6
发表时间：
1987
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
J. Evans;Y. Leblanc;B. Fitzsimmons;S. Charleson;D. Nathaniel;C. Léveillé
通讯作者：
C. Léveillé
血小板衍生生长因子增强主动脉平滑肌细胞中 12-L-羟基-5,8,10,14-二十碳四烯酸的内源性产生。
影响因子：
6.1
作者：
J. Nakao;Y. Koshihara;H. Ito;S. Murota;W. Chang
通讯作者：
W. Chang
吲哚美辛、乙炔酸、15-HETE、去甲二氢愈创木酸和 BW755C 对人白细胞和血小板中花生四烯酸代谢的影响比较。
DOI：
--
发表时间：
1984
期刊：
Prostaglandins Leukotrienes and Medicine
影响因子：
--
作者：
Hassan Salari;Pierre Braquet;P. Borgeat
通讯作者：
P. Borgeat
单羟基二十碳四烯酸的制备和测定。
影响因子：
2.9
作者：
Boeynaems,JM;Brash,AR;Oates,JA;Hubbard,WC
通讯作者：
Hubbard,WC