A 13CO2 breath test to assess the metabolism of triglyceride-rich lipoprotein remnants in mice.
A 13CO2 breath test to assess the metabolism of triglyceride-rich lipoprotein remnants in mice.
复制标题
13CO2 呼吸测试可评估小鼠体内富含甘油三酯的脂蛋白残余物的代谢。
DOI：
--
复制
发表时间：
1998
影响因子：
6.5
通讯作者：
T. Redgrave
中科院分区：
文献类型：
--
作者：
I. Martins;T. Redgrave
剩余物状乳剂是用类似于富含三酰甘油的脂蛋白的残留物的脂质组成制备的。当将标记有胆固醇[13C]油酸酯的残留物状乳剂注射到清醒小鼠的血液中时，这些乳剂在肝脏中代谢，并测量呼吸中13CO2的出现。在注射含有胆固醇[13C]油酸酯标记的残余物样乳剂的对照小鼠中，呼吸中13CO2的富集在45分钟达到峰值，然后在3小时内显著下降。apoe缺乏（-/-）小鼠中没有发现富集，低密度脂蛋白受体（-/-）缺乏（-/-）小鼠呼吸中13CO2的出现明显减少。这些发现与呼吸试验检测残余代谢缺陷的能力一致。呼吸试验可用于检测LDLr杂合子（+/-）小鼠的残余代谢缺陷，其中呼吸中13CO2的出现比对照小鼠少45分钟，但在实验期间仍保持升高。肝脂酶缺陷（-/-）小鼠未发现残余代谢缺陷。在禁食条件下，与进食小鼠相比，注射乳剂后呼吸中13CO2的富集量明显增加，说明在进食条件下，注射的残余物样乳剂可能被餐后颗粒代谢竞争。我们的研究结果表明，13C呼吸测试可以用来评估残留物的代谢。该方法为实验动物体内残余代谢的无创检测提供了一种实用、灵敏的方法。
Remnant-like emulsions were prepared with lipid compositions similar to remnants derived from triacylglycerol-rich lipoproteins. When injected into the bloodstream of conscious mice the remnant-like emulsions labeled with cholesteryl[13C]oleate were metabolized in the liver and the appearance of 13CO2 in the breath was measured. In control mice injected with remnant-like emulsions labeled with cholesteryl[13C]oleate, enrichment of 13CO2 in the breath peaked at 45 min and then decreased markedly by 3 h. In apoE-deficient (-/-) mice no enrichment was found and in low density lipoprotein receptor (LDLr)-deficient (-/-) mice the appearance of 13CO2 in the breath was markedly decreased. These findings were consistent with the ability of the breath test to detect defects in remnant metabolism. The breath test was useful in detecting a defect in remnant metabolism in LDLr heterozygote (+/-) mice, in which the appearance of 13CO2 in the breath was less by 45 min but remained elevated for the duration of the experiment when compared with control mice. In hepatic lipase-deficient (-/-) mice no defect in remnant metabolism was found. Under fasting conditions, the enrichment of 13CO2 in the breath after injection of emulsion was markedly increased when compared with fed mice, indicating that the metabolism of the injected remnant-like emulsion was probably competed for by post-prandial particles under fed conditions. Our findings show that a 13C breath test can be used to assess the metabolism of remnants. The test provides a useful and sensitive method for non-invasive testing of remnant metabolism in experimental animals.
登录
查看更多内容
肝脂肪酶在大鼠肝脏中乳糜微粒残留物的摄取和处理中的作用。
影响因子：
6.5
作者：
Shafi,S;Brady,SE;Bensadoun,A;Havel,RJ
通讯作者：
Havel,RJ
培养物中分泌肝脂肪酶的肝癌细胞增强残余脂蛋白的结合和摄取。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ji,ZS;Lauer,SJ;Fazio,S;Bensadoun,A;Taylor,JM;Mahley,RW
通讯作者：
Mahley,RW
甾醇侧链长度和结构影响大鼠和小鼠中乳糜微粒样脂肪乳剂的清除率。
影响因子：
6.5
作者：
Martins,IJ;Vilchèze,C;Mortimer,BC;Bittman,R;Redgrave,TG
通讯作者：
Redgrave,TG
DOI：
10.1016/s1388-1981(03)00087-8
发表时间：
2003-07-04
影响因子：
4.8
作者：
Fezza, F;Dillwith, JW;Sauer, JR
通讯作者：
Sauer, JR