Photodynamic inactivation of Acinetobacter baumannii using phenothiazinium dyes: in vitro and in vivo studies.
Photodynamic inactivation of Acinetobacter baumannii using phenothiazinium dyes: in vitro and in vivo studies.
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DOI：
10.1002/lsm.20922
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发表时间：
2010-07
影响因子：
2.4
通讯作者：
Hamblin, Michael R.
中科院分区：
文献类型：
--
作者：
Ragas, Xavier;Dai, Tianhong;Tegos, George P.;Agut, Montserrat;Nonell, Santi;Hamblin, Michael R.
吩噻嗪鎓染料已被报道是有效的光敏剂，在用红光照射后在体外灭活广泛的微生物。然而，它们在体内的应用尚未被广泛探索。本研究评价吩噻嗪染料对多重耐药鲍曼不动杆菌的体外和体内杀菌活性。我们报道了甲苯胺蓝O、亚甲蓝、1，9-二甲基亚甲蓝和新亚甲蓝对多药耐药铜绿假单胞菌的光动力灭活研究。体外鲍曼不动杆菌。选择最有效的染料进行体内研究，使用感染生物发光A的三度小鼠烧伤。鲍曼不动杆菌菌株，用652 nm非相干光源照射。每天对小鼠进行成像，持续2周，以观察光动力疗法（PDT）治疗的小鼠与未治疗的烧伤小鼠之间的生物发光-时间曲线的差异。所有染料在体外均对A.在30 J/cm 2照射635或652 nm红光后，当染料保留在溶液中时，具有更有效的杀伤作用。新亚甲蓝是四种染料中最有效的，在360 J/cm 2和800 μM染料剂量后，体内PDT期间细菌发光降低3.2-log。此外，观察到PDT处理的小鼠的生物发光-时间曲线下的面积的统计学显著减少，表明PDT后感染没有复发。吩噻嗪染料，特别是新的亚甲蓝，是PDT治疗多药耐药A。体内鲍曼不动杆菌。
Phenothiazinium dyes have been reported to be effective photosensitizers inactivating a wide range of microorganisms in vitro after illumination with red light. However, their application in vivo has not extensively been explored. This study evaluates the bactericidal activity of phenothiazinium dyes against multidrug-resistant Acinetobacter baumannii both in vitro and in vivo. We report the investigation of toluidine blue O, methylene blue, 1,9-dimethylmethylene blue, and new methylene blue for photodynamic inactivation of multidrug-resistant A. baumannii in vitro. The most effective dye was selected to carry out in vivo studies using third-degree mouse burns infected with a bioluminescent A. baumannii strain, upon irradiation with a 652 nm noncoherent light source. The mice were imaged daily for 2 weeks to observe differences in the bioluminescence–time curve between the photodynamic therapy (PDT)-treated mice in comparison with untreated burns. All the dyes were effective in vitro against A. baumannii after 30 J/cm2 irradiation of 635 or 652 nm red light had been delivered, with more effective killing when the dye remained in solution. New methylene blue was the most effective of the four dyes, achieving a 3.2-log reduction of the bacterial luminescence during PDT in vivo after 360 J/cm2 and an 800 μM dye dose. Moreover, a statistically significant reduction of the area under the bioluminescence–time curve of PDT-treated mice was observed showing that the infection did not recur after PDT. Phenothiazinium dyes, and especially new methylene blue, are potential photosensitizers for PDT to treat burns infected with multidrug-resistant A. baumannii in vivo.
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影响因子：
4.9
作者：
Tegos, GP;Anbe, M;Hamblin, MR
通讯作者：
Hamblin, MR
影响因子：
5.2
作者：
Hamblin, MR;O'Donnell, DA;Hasan, T
通讯作者：
Hasan, T
影响因子：
4.9
作者：
Dai, Tianhong;Tegos, George P.;Hamblin, Michael R.
通讯作者：
Hamblin, Michael R.
影响因子：
2.7
作者：
Gilpin, DA
通讯作者：
Gilpin, DA
DOI：
10.1016/1011-1344(95)07148-2
发表时间：
1996-02-01
影响因子：
5.4
作者：
Minnock, A;Vernon, DI;Brown, SB
通讯作者：
Brown, SB