Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion.
Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion.
复制标题
DOI：
10.1016/j.jsb.2018.02.001
复制
发表时间：
2018-06
影响因子：
3
通讯作者：
Agard DA
中科院分区：
文献类型：
--
作者：
Fernandez JJ;Li S;Bharat TAM;Agard DA
Recent evidence suggests that the beam-induced motion of the sample during tilt-series acquisition is a major resolution-limiting factor in electron cryo-tomography (cryoET). It causes suboptimal tilt-series alignment and thus deterioration of the reconstruction quality. Here we present a novel approach to tilt-series alignment and tomographic reconstruction that considers the beam-induced sample motion through the tilt-series. It extends the standard fiducial-based alignment approach in cryoET by introducing quadratic polynomials to model the sample motion. The model can be used during reconstruction to yield a motion-compensated tomogram. We evaluated our method on various datasets with different sample sizes. The results demonstrate that our method could be a useful tool to improve the quality of tomograms and the resolution in cryoET.
登录
查看更多内容
影响因子：
2.2
作者：
Fernandez, J. J.;Li, S.;Crowther, R. A.
通讯作者：
Crowther, R. A.
影响因子：
3
作者：
Kremer, JR;Mastronarde, DN;McIntosh, JR
通讯作者：
McIntosh, JR
影响因子：
3
作者：
Agulleiro, Jose-Ignacio;Fernandez, Jose-Jesus
通讯作者：
Fernandez, Jose-Jesus
一种基于尺度不变特征的无标记自动对齐方法
影响因子：
3
作者：
Han, Renmin;Zhang, Fa;Liu, Zhiyong
通讯作者：
Liu, Zhiyong
影响因子：
2.2
作者：
Chen, Shaoxia;McMullan, Greg;Faruqi, Abdul R.;Murshudov, Garib N.;Short, Judith M.;Scheres, Sjors H. W.;Henderson, Richard
通讯作者：
Henderson, Richard