Sulforaphane absorption and histone deacetylase activity following single dosing of broccoli sprout supplement in normal dogs.
Sulforaphane absorption and histone deacetylase activity following single dosing of broccoli sprout supplement in normal dogs.
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DOI：
10.1002/vms3.118
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发表时间：
2018-11
影响因子：
1.7
通讯作者：
Ho E
中科院分区：
文献类型：
--
作者：
Curran KM;Bracha S;Wong CP;Beaver LM;Stevens JF;Ho E
表观遗传改变在癌症中的作用得到了越来越多的关注，并导致我们对导致癌症易感性的机制的理解发生了范式转变。萝卜硫素（SFN）是一种天然存在的异硫氰酸盐，它是从硫代葡萄糖苷前体硫代葡萄糖苷（GFN）中提取出来的，硫代葡萄糖苷存在于十字花科蔬菜中，如西兰花。萝卜硫素通过抑制组蛋白去乙酰化酶等多种机制抑制肿瘤生长。这项研究的目的是提供一个详细的分析萝卜硫素吸收后，单剂量的西兰花芽补充剂在正常的狗。给10只健康成年犬口服单剂量西兰花芽补充剂（含活性黑芥子酶）。在给药前和给药后的不同时间点采集血液和尿液样本。血浆总SFN代谢物水平在食用后4小时达到峰值，并在食用后24小时被清除。尿SFN代谢物在补充后4小时达到峰值，在补充后24和48小时仍可检测到。在食用后1小时观察到组蛋白去乙酰化酶活性下降的趋势，在食用后24小时观察到显著下降。本研究的数据表明，口服SFN可被狗吸收，给药后可在血浆和尿液中检测到SFN代谢物，并且单次给药后SFN及其代谢物对组蛋白去乙酰化酶活性有一定影响。
The role of epigenetic alterations during cancer has gained increasing attention and has resulted in a paradigm shift in our understanding of mechanisms leading to cancer susceptibility. Sulforaphane (SFN) is a naturally occurring isothiocyanate derived from the precursor glucosinolate, glucoraphanin (GFN), which is found in cruciferous vegetables such as broccoli. Sulforaphane has been shown to suppress tumour growth by several mechanisms including inhibiting histone deacetylases. The objective of this study was to provide a detailed analysis of sulforaphane absorption following a single oral dose of a broccoli sprout supplement in normal dogs. A single dose of broccoli sprout supplement (with active myrosinase) was orally administered to 10 healthy adult dogs. Blood and urine samples were collected prior to dosing, and at various time points post‐dosing. Plasma total SFN metabolite levels peaked at 4 h post‐consumption and were cleared by 24 h post‐consumption. Urinary SFN metabolites peaked at 4 h post‐consumption, and remained detectable at 24 and 48 h post‐supplement consumption. A trend for decrease in histone deacetylase activity was observed at 1 h post‐consumption and a significant decrease was observed at 24 h post‐consumption. The data presented herein indicate that oral SFN is absorbed in dogs, SFN metabolites are detectable in plasma and urine post‐dosing, and SFN and its metabolites have some effect on histone deacetylase activity following a single dose.
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影响因子：
5.2
作者：
Clarke, John D.;Hsu, Anna;Yu, Zhen;Dashwood, Roderick H.;Ho, Emily
通讯作者：
Ho, Emily
影响因子：
5.7
作者：
Hsu A;Wong CP;Yu Z;Williams DE;Dashwood RH;Ho E
通讯作者：
Ho E
影响因子：
2.1
作者：
通讯作者：
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影响因子：
6.1
作者：
Clarke JD;Riedl K;Bella D;Schwartz SJ;Stevens JF;Ho E
通讯作者：
Ho E
DOI：
10.1158/1940-6207.capr-15-0119
发表时间：
2015-12
期刊：
Cancer prevention research (Philadelphia, Pa.)
影响因子：
--
作者：
Atwell LL;Zhang Z;Mori M;Farris P;Vetto JT;Naik AM;Oh KY;Thuillier P;Ho E;Shannon J
通讯作者：
Shannon J