Layered patterning of hepatocytes in co-culture systems using microfabricated stencils.
Layered patterning of hepatocytes in co-culture systems using microfabricated stencils.
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DOI：
10.2144/000113317
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发表时间：
2010-01
期刊：
影响因子：
2.7
通讯作者：
Yarmush ML
中科院分区：
文献类型：
--
作者：
Cho CH;Park J;Tilles AW;Berthiaume F;Toner M;Yarmush ML
组织工程中的微制造和微图案化技术为创造和控制可以观察细胞行为的微环境提供了巨大的潜力。在这里，我们提出了一种新的方法，利用微制聚二甲基硅氧烷(PDMS)模板在微图案化的成纤维细胞饲养层上生成肝细胞的分层图案化。我们制作了直径为500微米的PDMS模板，将肝细胞与3T3-J2成纤维细胞以两种模式进行共培养，以评估和表征共培养系统中细胞的相互作用。本研究的结果表明，当肝细胞和成纤维细胞之间的异型界面被增加时，细胞内白蛋白染色和E-钙粘素表达均匀，肝脏特异性功能增强，肝细胞糖原合成活跃。这种图案化技术在基础科学、药物筛选和组织工程中的应用以及生物人工肝装置的设计中都是一种有用的实验工具。
Microfabrication and micropatterning techniques in tissue engineering offer great potential for creating and controlling microenvironments in which cell behavior can be observed. Here we present a novel approach to generate layered patterning of hepatocytes on micropatterned fibroblast feeder layers using microfabricated polydimethylsiloxane (PDMS) stencils. We fabricated PDMS stencils to pattern circular holes with diameters of 500 µm. Hepatocytes were co-cultured with 3T3-J2 fibroblasts in two types of patterns to evaluate and characterize the cellular interactions in the co-culture systems. Results of this study demonstrated uniform intracellular albumin staining and E-cadherin expression, increased liver-specific functions, and active glycogen synthesis in the hepatocytes when the heterotypic interface between hepatocytes and fibroblasts was increased by the layered patterning technique. This patterning technique can be a useful experimental tool for applications in basic science, drug screening, and tissue engineering, as well as in the design of bioartificial liver devices.
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影响因子：
6.1
作者：
Park, Jaesung;Cho, Cheul H.;Yarmush, Martin L.
通讯作者：
Yarmush, Martin L.
影响因子：
3.8
作者：
Cho, Chenl H.;Berthiaume, Francois;Tilles, Arno W.;Yarmush, Martin L.
通讯作者：
Yarmush, Martin L.
影响因子：
15.9
作者：
BISSELL, DM;ARENSON, DM;ROLL, FJ
通讯作者：
ROLL, FJ
影响因子：
11.5
作者：
Nakajima, S;Doi, R;Imamura, M
通讯作者：
Imamura, M
影响因子：
82.9
作者：
Ohashi, Kazuo;Yokoyama, Takashi;Nakajima, Yoshiyuki
通讯作者：
Nakajima, Yoshiyuki