Capillary dilation and rarefaction are correlated with intracapillary inflammation in antibody-mediated rejection.
Capillary dilation and rarefaction are correlated with intracapillary inflammation in antibody-mediated rejection.
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DOI：
10.1155/2014/582902
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发表时间：
2014
影响因子：
4.1
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Li X;Sun Q;Zhang M;Xie K;Chen J;Liu Z
抗体介导的排斥反应(ABMR)仍然是肾移植术后移植物丢失的主要原因之一。它以微循环中的内皮损伤为主。阐明微循环损害的机制显然是理解ABMR发病机制的关键一步。在这里，我们描述了ABMR中的毛细血管变异及其可能的机制。与T细胞介导的排斥反应和稳定的移植物相比，ABMR组肾小管周围毛细血管(PTCs)明显扩张和疏松；Image-Pro Plus显示PTC内面积显著增大。有趣的是，PTC的扩张与PTC内细胞计数密切相关。此外，肾小管周围毛细血管炎症与T-bet的原位表达呈正相关，且T-bet在PTC内的表达与PTC直径呈正相关。缺氧诱导因子-1α表达上调对毛细血管扩张无明显影响。总体而言，ABMR的特点是早期毛细血管扩张和稀疏；我们的数据证实，这种扩张与毛细血管内炎症密切相关，而毛细血管炎症又与原位T-bet表达相关。T-bet在微循环损伤的发生发展中起重要作用，是治疗ABMR的潜在靶点。
Antibody-mediated rejection (ABMR) remains one of the major causes of graft loss after renal transplantation. It is dominated by endothelial damage in microcirculation. Clarifying the mechanism of microcirculating damage is obviously a key step to understand the pathogenesis of ABMR. Here we characterized capillary variation in ABMR and its possible mechanisms. Compared with T cell-mediated rejection and stable grafts, there was a significant dilation and rarefaction in peritubular capillaries (PTCs) of the ABMR group; Image-Pro Plus revealed a significantly larger intra-PTC area. Interestingly, the dilation of PTCs was strongly correlated with the intra-PTC cell counting. Moreover, peritubular capillary inflammation is correlated with in situ T-bet expression, and there was a good correlation between the intra-PTC expression of T-bet and the PTC diameter. HIF-1α up-regulation could be observed in ABMR but it was not necessary for capillary dilation. In general, ABMR is characterized with early capillary dilation and rarefaction; our data confirmed that the dilation is strongly correlated with intracapillary inflammation, which in turn is correlated with in situ T-bet expression. T-bet plays an important role in the development of microcirculating injury, and thus it is a potential target for the treatment of ABMR.
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影响因子：
8.8
作者：
Gibson, I. W.;Gwinner, W.;Mengel, M.
通讯作者：
Mengel, M.
影响因子：
2.2
作者：
Hillen, Femke;van de Winkel, Anouk;Griffioen, Arjan W.
通讯作者：
Griffioen, Arjan W.
影响因子：
19.6
作者：
Magil, AB;Tinckam, K
通讯作者：
Tinckam, K
影响因子：
0.8
作者：
Kulkarni P;Uppin MS;Prayaga AK;Das U;Dakshinamurthy KV
通讯作者：
Dakshinamurthy KV
影响因子：
8.8
作者：
Sis, Banu;Jhangri, Gian S.;Halloran, Philip F.
通讯作者：
Halloran, Philip F.