MiR-30a inhibits BECN1-mediated autophagy in diabetic cataract.
MiR-30a inhibits BECN1-mediated autophagy in diabetic cataract.
复制标题
MiR-30a 抑制糖尿病白内障中 BECN1 介导的自噬
DOI：
10.18632/oncotarget.20483
复制
发表时间：
2017-09-29
期刊：
影响因子：
--
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Zhang L;Cheng R;Huang Y
目的探讨microrna在糖尿病性白内障形成过程中对晶状体上皮细胞（LECs）自噬和凋亡的调控作用。方法采用miRNA微阵列技术和实时荧光定量PCR技术检测糖尿病性白内障lec中miRNA的表达。在高糖条件下培养人LECs作为糖尿病性白内障模型。采用Western blotting和实时荧光定量PCR检测BECN1和LC3B。流式细胞术检测细胞凋亡程度。结果在糖尿病性白内障组织附着的LECs中发现miR-30a下调。通过生物信息学实验和荧光素酶活性实验发现BECN1是miR-30a的直接靶点。MiR-30a在体外降低becn1介导的高糖诱导的LECs自噬活性。细胞凋亡比例明显降低。结论MiR-30a通过靶向BECN1参与糖尿病性白内障LECs自噬的抑制。
Purpose To investigate the role of microRNAs in the regulation of autophagy and apoptosis in lens epithelial cells (LECs) during diabetic cataract formation. Methods A miRNA microarray study and quantitative real-time PCR were performed to identify the expression of miRNAs in LECs of diabetic cataract. Human LECs were cultured in high glucose conditions as a diabetic cataract model. BECN1 and LC3B were detected by Western blotting and quantitative real-time PCR. The extent of apoptosis was measured using FACSCalibur flow cytometry. Results Downregulation of miR-30a was identified in LECs attached to diabetic cataract tissues. By the bioinformatic assay and the luciferase activity assay, BECN1 was found to be a direct target of miR-30a. MiR-30a reduced the BECN1-mediated autophagy activity induced by high glucose in LECs in vitro. The ratio of LECs apoptosis was also decreased. Conclusion MiR-30a was involved in the inhibition of autophagy by targeting BECN1 in LECs in human diabetic cataract.
登录
查看更多内容
影响因子：
3.5
作者：
Harries LW
通讯作者：
Harries LW
Micro-RNA-30a 通过靶向原代培养的皮质神经元和中风后小鼠大脑中的热休克蛋白 HSPA5 来调节缺血诱导的细胞死亡
影响因子：
4.2
作者：
Wang, Peng;Zhang, Nan;Li, Junfa
通讯作者：
Li, Junfa
Atg7 调节 p53 活性以调节代谢应激期间的细胞周期和存活
DOI：
10.1126/science.1218395
发表时间：
2012-04-13
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Lee IH;Kawai Y;Fergusson MM;Rovira II;Bishop AJ;Motoyama N;Cao L;Finkel T
通讯作者：
Finkel T
影响因子：
64.8
作者：
Tanaka, H;Arakawa, H;Nakamura, Y
通讯作者：
Nakamura, Y
影响因子：
3.4
作者：
Dong, Yuchen;Zheng, Yajuan;Zhao, Meisheng
通讯作者：
Zhao, Meisheng