Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion.
Cell Type Variability in the Incorporation of Lipids in the Dengue Virus Virion.
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DOI：
10.3390/v14112566
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发表时间：
2022-11-19
期刊：
影响因子：
--
通讯作者：
Smith DR
中科院分区：
文献类型：
--
作者：
Hitakarun A;Williamson MK;Yimpring N;Sornjai W;Wikan N;Arthur CJ;Pompon J;Davidson AD;Smith DR
由宿主膜产生的脂质双层约占登革热病毒 (DENV) 病毒颗粒重量的 20%，对于病毒进入至关重要。尽管病毒粒子的脂质成分很重要，但人们对它的了解仍然知之甚少。与用于生成病毒粒子的细胞的脂质谱相结合，这项工作确定了源自两种细胞系（C6/36 和 LLC-MK2）的 DENV 病毒粒子的部分脂质谱。结果显示两种细胞类型之间存在独特的特征。在哺乳动物 LLC-MK2 细胞中，30.8%（73/237 种已识别的脂质种类；31 种上调，42 种下调）的脂质种类因感染而发生变化，而在昆虫 C6/36 细胞中，只有 12.0%（25/208；19 种上调，6 种下调）脂质种类因感染而发生变化。对于来自 LLC-MK2 细胞的病毒粒子，在病毒粒子中专门检测到 14 种脂质，另外还有 7 种脂质被富集（超过模拟对照）。对于来自 C6/36 细胞的病毒体，检测到 43 种脂质，这些脂质在模拟制剂中未见，另外 16 种脂质被特异性富集（超过模拟对照）。这些结果提供了对哺乳动物和蚊子细胞中产生的 DENV 病毒粒子的首次脂质描述，以及相应感染细胞中的脂质变化。
A lipid bilayer produced from the host membrane makes up around 20% of the weight of the dengue virus (DENV) virion and is crucial for virus entry. Despite its significance, the virion’s lipid composition is still poorly understood. In tandem with lipid profiles of the cells utilised to generate the virions, this work determined a partial lipid profile of DENV virions derived from two cell lines (C6/36 and LLC-MK2). The results showed distinctive profiles between the two cell types. In the mammalian LLC-MK2 cells, 30.8% (73/237 identified lipid species; 31 upregulated, 42 downregulated) of lipid species were altered in response to infection, whilst in insect C6/36 cells only 12.0% (25/208; 19 upregulated, 6 downregulated) of lipid species showed alterations in response to infection. For virions from LLC-MK2 cells, 14 lipids were detected specifically in virions with a further seven lipids being enriched (over mock controls). For virions from C6/36 cells, 43 lipids were detected that were not seen in mock preparations, with a further 16 being specifically enriched (over mock control). These results provide the first lipid description of DENV virions produced in mammalian and mosquito cells, as well as the lipid changes in the corresponding infected cells.
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影响因子：
6.7
作者：
Chotiwan N;Andre BG;Sanchez-Vargas I;Islam MN;Grabowski JM;Hopf-Jannasch A;Gough E;Nakayasu E;Blair CD;Belisle JT;Hill CA;Kuhn RJ;Perera R
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DOI：
10.1126/science.aaa8651
发表时间：
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期刊：
Science (New York, N.Y.)
影响因子：
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影响因子：
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作者：
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Mackenzie, Jason M.
DOI：
10.15585/mmwr.rr7006a1
发表时间：
2021-12-17
期刊：
MMWR. Recommendations and reports : Morbidity and mortality weekly report. Recommendations and reports
影响因子：
--
作者：
Paz-Bailey G;Adams L;Wong JM;Poehling KA;Chen WH;McNally V;Atmar RL;Waterman SH
通讯作者：
Waterman SH