Cell-specific transfection of choriocarcinoma cells by using Sindbis virus hCG expressing chimeric vector.
Cell-specific transfection of choriocarcinoma cells by using Sindbis virus hCG expressing chimeric vector.
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使用表达辛德比斯病毒 hCG 的嵌合载体对绒毛膜癌细胞进行细胞特异性转染。
DOI：
10.1006/bbrc.1998.8922
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Meruelo,D
中科院分区：
文献类型：
--
作者：
Sawai,K;Meruelo,D
The development of Sindbis virus vectors that can target specific cell types would provide an important gene therapy strategy. We explored the possibility of designing a Sindbis virus vector that can target human choriocarcinoma cells via ligand-receptor interaction. The Sindbis virus envelope gene was modified by insertion of the α- and β-hCG genes. The chimeric helper RNA was then transfected into BHK cells along with a virus-based expression vector, allowing the production of virus particles containing hCG-envelope chimeras. The hCG-envelope chimeric virus vector has minimal infectivities against BHK cells and human cancer cells which do not contain LH/CG receptors on their surface. This vector can, however, infect and transfer a reporter gene to choriocarcinoma cells as well as other cells bearing LH/CG receptors. This chimeric Sindbis virus vector may provide a novel approach for gene therapy of gestational trophoblast disease and placental dysfunction.
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DOI：
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发表时间：
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影响因子：
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DOI：
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