Organic Anion Transporting Polypeptide 1B1 Is a Potential Reporter for Dual MR and Optical Imaging.
Organic Anion Transporting Polypeptide 1B1 Is a Potential Reporter for Dual MR and Optical Imaging.
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DOI：
10.3390/ijms22168797
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发表时间：
2021-08-16
影响因子：
5.6
通讯作者：
Hsiao JK
中科院分区：
文献类型：
--
作者：
Lee YH;Wu MR;Hsiao JK
负责运输磁共振（MR）和荧光造影剂的膜蛋白特别重要，因为它们是非侵入性分子成像中潜在的报告蛋白。钆贝酸二葡胺 (Gd-BOPTA) 是一种肝脏特异性 MR 造影剂，已在全球使用 10 多年。然而，相应的分子运输机制尚未得到验证。我们之前报道过有机阴离子转运多肽 (OATP) 1B3 对 MR 试剂 (Gd-EOB-DTPA) 和临床可用的近红外 (NIR) 荧光染料吲哚菁绿 (ICG) 具有摄取能力。本研究进一步评估了 OATP 家族的另一种多肽 OATP1B1，以确定其报告能力。在OATP1B1转染的293T瞬时表达模型中，通过1.5T MR成像证实了Gd-BOPTA和Gd-EOB-DTPA的摄取。在 HT-1080 细胞系的恒定 OAPT1B1 和 OATP1B3 表达模型中，观察到 HT-1080-OAPT1B1 和 HT-1080-OATP1B3 摄取 Gd-BOPTA 和 Gd-EOB-DTPA。最后，我们验证了 OATP1B1 和 OATP1B3 的 ICG 摄取能力。 OAPT1B3 表现出优于 OAPT1B1 的 ICG 摄取能力。我们得出结论，OATP1B1 是双 MR 和 NIR 荧光分子成像的潜在报告基因，特别是与 Gd-BOPTA 结合使用。
Membrane proteins responsible for transporting magnetic resonance (MR) and fluorescent contrast agents are of particular importance because they are potential reporter proteins in noninvasive molecular imaging. Gadobenate dimeglumine (Gd-BOPTA), a liver-specific MR contrast agent, has been used globally for more than 10 years. However, the corresponding molecular transportation mechanism has not been validated. We previously reported that the organic anion transporting polypeptide (OATP) 1B3 has an uptake capability for both MR agents (Gd-EOB-DTPA) and indocyanine green (ICG), a clinically available near-infrared (NIR) fluorescent dye. This study further evaluated OATP1B1, another polypeptide of the OATP family, to determine its reporter capability. In the OATP1B1 transfected 293T transient expression model, both Gd-BOPTA and Gd-EOB-DTPA uptake were confirmed through 1.5 T MR imaging. In the constant OAPT1B1 and OATP1B3 expression model in the HT-1080 cell line, both HT-1080-OAPT1B1 and HT-1080-OATP1B3 were observed to ingest Gd-BOPTA and Gd-EOB-DTPA. Lastly, we validated the ICG uptake capability of both OATP1B1 and OATP1B3. OAPT1B3 exhibited a superior ICG uptake capability to that of OAPT1B1. We conclude that OATP1B1 is a potential reporter for dual MR and NIR fluorescent molecular imaging, especially in conjunction with Gd-BOPTA.
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