Early inflammatory response to asbestos exposure in rat and hamster lungs: role of inducible nitric oxide synthase.
Early inflammatory response to asbestos exposure in rat and hamster lungs: role of inducible nitric oxide synthase.
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大鼠和仓鼠肺中石棉暴露的早期炎症反应：诱导型一氧化氮合酶的作用。
DOI：
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发表时间：
2002
影响因子：
3.8
通讯作者：
F. Krombach
中科院分区：
文献类型：
--
作者：
M. Dörger;A. Allmeling;R. Kiefmann;S. Münzing;K. Messmer;F. Krombach
最近的研究表明，诱导型一氧化氮合酶（iNOS）在石棉相关肺部疾病的发展中起作用。众所周知，暴露于石棉纤维的大鼠和仓鼠的肺反应是完全不同的。此外，体外实验表明，与大鼠相比，仓鼠的单核吞噬细胞缺乏iNOS途径。因此，本研究的目的是探讨石棉纤维暴露后大鼠和仓鼠体内肺iNOS表达是否存在差异，iNOS诱导的差异是否与急性肺部炎症反应的差异有关。测定石棉纤维气管内灌注1天和7 d后CD大鼠和叙利亚金仓鼠的体重、肺泡-动脉氧差、支气管肺泡灌洗液中细胞差数、总蛋白渗漏、肺髓过氧化物酶活性和脂质过氧化、干湿比、iNOS mRNA和蛋白表达以及肺组织硝基酪氨酸染色。暴露于石棉纤维的大鼠导致肺iNOS表达和硝基酪氨酸染色增强，并伴有急性炎症，其特征是中性粒细胞涌入，髓过氧化物酶活性增强和脂质过氧化，肺泡毛细血管膜损伤，水肿形成和气体交换受损。相比之下，在仓鼠中灌注石棉纤维导致的肺部炎症反应明显较轻，肺细胞中没有诱导iNOS。所获得的数据为了解暴露于石棉纤维后肺反应的物种差异的潜在机制提供了重要信息。
Recent studies have suggested that inducible nitric oxide synthase (iNOS) plays a role in the development of asbestos-related pulmonary disorders. The pulmonary reactions of rats and hamsters upon exposure to asbestos fibers are well known to be disparate. In addition, in vitro experiments have indicated that mononuclear phagocytes from hamsters, in contrast to those from rats, lack the iNOS pathway. Therefore, the purpose of this study was to investigate whether rats and hamsters differ in lung iNOS expression in vivo upon exposure to asbestos fibers and whether differences in iNOS induction are associated with differences in the acute pulmonary inflammatory reaction. Body weight, alveolar-arterial oxygen difference, differential cell count in bronchoalveolar lavage fluid, total protein leakage, lung myeloperoxidase activity and lipidperoxidation, wet/dry ratio, iNOS mRNA and protein expression, and nitrotyrosine staining of lung tissue were determined 1 and 7 days after intratracheal instillation of asbestos fibers in CD rats and Syrian golden hamsters. Exposure of rats to asbestos fibers resulted in enhanced pulmonary iNOS expression and nitrotyrosine staining together with an acute inflammation that was characterized by an influx of neutrophils, enhanced myeloperoxidase activity and lipid peroxidation, damage of the alveolar-capillary membrane, edema formation, and impairment of gas exchange. In comparison, instillation of asbestos fibers in hamsters resulted in a significantly milder inflammatory reaction of the lung with no induction of iNOS in pulmonary cells. The data obtained provide important information to understand the underlying mechanisms of species differences in the pulmonary response upon exposure to asbestos fibers.
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DOI：
10.1152/jappl.1995.78.1.164
发表时间：
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期刊：
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影响因子：
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1995-02
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DOI：
10.1152/ajplung.1999.276.2.l263
发表时间：
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期刊：
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影响因子：
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