UHPLC-PDA-ESI/HRMSn profiling method to identify and quantify oligomeric proanthocyanidins in plant products.
UHPLC-PDA-ESI/HRMSn profiling method to identify and quantify oligomeric proanthocyanidins in plant products.
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DOI：
10.1021/jf501011y
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发表时间：
2014-10-01
影响因子：
6.1
通讯作者：
Harnly JM
中科院分区：
文献类型：
--
作者：
Lin LZ;Sun J;Chen P;Monagas MJ;Harnly JM
通过 UHPLC-PDA-HRMSn 成功鉴定了一系列植物源材料（枣子、富士苹果、荔枝和山竹果皮、黑巧克力、葡萄籽和蔓越莓提取物）中的低聚原花青素。通过根据聚合度、flavan-3-ol 成分以及 A 型键和没食子酰基的数量计算高精度质量，从理论上预测了 247 种原花青素的身份。 MSn 片段允许根据 A 型键的单体、连接性和位置对 flavan-3-ol 进行表征。根据理论计算对50种PA的双电荷或三电荷离子进行了鉴定。使用单一儿茶素标准品和摩尔相对响应因子 (MRRF) 来量化分离良好的 PA。 SIM 峰计数的比率用于量化每种未分离的异构体。这是直接测定植物源性食品中每种原花青素和葡萄籽中含有表菲瑟替尼醇单元的原花青素的第一份报告。
Oligomeric proanthocyanidins were successfully identified by UHPLC-PDA-HRMSn in a selection of plant-derived materials (jujube fruit, Fuji apple, fruit pericarps of litchi and mangosteen, dark chocolate, and grape seed and cranberry extracts). The identities of 247 proanthocyanidins were theoretically predicted by computing high-accuracy masses based on the degree of polymerization, flavan-3-ol components, and the number of A type linkages and galloyls. MSn fragments allowed characterization on flavan-3-ol based on the monomer, connectivity, and location of A-type bonds. Identification of doubly or triply charged ions of 50 PAs was made on the basis of theoretical calculations. A single catechin standard and molar relative response factors (MRRFs) were used to quantify the well-separated PAs. The ratios of the SIM peak counts were used to quantify each of the unseparated isomers. This is the first report of direct determination of each of the proanthocyanidins in plant-derived foods and proanthocyanidins containing an epifisetinidol unit in grape seeds.
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影响因子：
6.1
作者：
Lin LZ;Sun J;Chen P;Harnly J
通讯作者：
Harnly J
影响因子：
6.1
作者：
Hellstrom, Jarkko K.;Torronen, A. Riitta;Mattila, Pirjo H.
通讯作者：
Mattila, Pirjo H.
影响因子：
6.1
作者：
Monagas, M;Gómez-Cordovés, C;Ricardo-Da-Silva, JM
通讯作者：
Ricardo-Da-Silva, JM
影响因子：
6.1
作者：
Cooper, Karen A.;Campos-Gimenez, Esther;Williamson, Gary
通讯作者：
Williamson, Gary
影响因子：
4.1
作者：
Núñez, V;Gómez-Cordovés, C;Mitchell, AE
通讯作者：
Mitchell, AE