A review of methodology used to measure leaf litter decomposition in lotic environments: Time to turn over an old leaf?
A review of methodology used to measure leaf litter decomposition in lotic environments: Time to turn over an old leaf?
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对用于测量潮气环境中落叶分解的方法的回顾：是时候翻动旧叶子了？
DOI：
10.1071/mf9910001
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发表时间：
1991
影响因子：
1.8
通讯作者：
P. Boon
中科院分区：
文献类型：
--
作者：
A. Boulton;P. Boon
尽管认识到微生物调节和无脊椎动物摄食之间的关系，微生物生态学家和动物学家之间的沟通很少研究流中落叶的处理。对合适的方法进行评估是及时的，以鼓励工作人员批判性地检查他们的实验方法：人们经常忘记，结果在很大程度上取决于所使用的方法。我们回顾了最近的一些研究，强调微生物和生物化学方法的应用，并讨论了一些重要的决定，必须在实验设计和实施。建议在试图模拟自然树叶分解的研究中使用新落的自然脱落的树叶，将其捆绑在树叶包中并拴在自然基质上。应避免在极端条件下干燥和浸滤树叶。质量损失不应简单地等同于分解;相反，应量化植物凋落物主要化学成分类型的损失。如果目的是研究分解，而不仅仅是叶片分解，溶解物质和细颗粒物质从腐烂的叶子失去的代谢也必须解决。应使用适当的技术来研究微生物组合：脂质生物标志物或核酸方法用于组合组成，显微镜或生物化学分析用于微生物生物量，[3 H]-胸苷或[3 H]-亮氨酸方法用于确定生长速率。应谨慎使用指数衰减曲线和处理系数，特别是在整个研究中样本量较小且相等的情况下。
Despite the recognition of the relationship between microbial conditioning and invertebrate feeding, there has been little communication between microbial ecologists and zoologists studying the processing of leaf litter in streams. An appraisal of suitable methods is timely, to encourage workers to critically examine their experimental approach: it is often forgotten that results are determined largely by the methods used. We review some recent studies, emphasizing the application of microbiological and biochemical methods, and discuss some important decisions that must be made in experimental design and implementation. It is recommended that newly fallen, naturally abscissed leaves, bound in leaf packs and tethered to natural substrata, be used in studies attempting to simulate natural leaf decomposition. Drying and leaching leaves under extreme conditions should be avoided. Mass loss should not simply be equated with decomposition; instead, losses of the main types of chemical constituents of plant litter should be quantified. If the aim is to study decomposition rather than merely leaf breakdown, the metabolism of dissolved matter and fine particulate material lost from the decaying leaf must be addressed as well. Appropriate techniques should be used to study microbial assemblages: lipid biomarkers or nucleic-acid methods for assemblage composition, microscopy or biochemical analysis for microbial biomass, [3H]-thymidine or [3H]-leucine methods to determine growth rates. Exponential decay curves and processing coefficients should be used cautiously, especially if sample sizes are small and equal throughout the study.
一种改进的气相色谱法，用于测量葡萄糖胺和胞壁酸浓度。
影响因子：
2.9
作者：
Hicks,RE;Newell,SY
通讯作者：
Newell,SY