Algae as protein factories: expression of a human antibody and the respective antigen in the diatom Phaeodactylum tricornutum.
Algae as protein factories: expression of a human antibody and the respective antigen in the diatom Phaeodactylum tricornutum.
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DOI：
10.1371/journal.pone.0028424
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Maier UG
中科院分区：
文献类型：
--
作者：
Hempel F;Lau J;Klingl A;Maier UG
微藻结合了高生长速率和真核表达系统的所有优点，被认为是各种工业、治疗和诊断重组蛋白的巨大潜力表达系统。此外，微藻表现出像陆地植物一样的光养生活方式，因此蛋白质的表达是由光合作用推动的，光合作用对二氧化碳是中性的，而且只涉及较低的生产成本。然而，到目前为止，用于表达重组蛋白的藻类生物反应器的研究非常少见，这需要在这个非常有前途的领域进行进一步的研究。在这项研究中，我们提供了抗乙肝表面蛋白和相应抗原的人单抗在三角褐指藻中的表达数据。抗体是完全组装和功能的，累积到总可溶性蛋白的8.7%，相当于每克藻类干重21毫克抗体。乙肝表面蛋白也具有功能，并被藻类产生的抗体和商业抗体识别。
Microalgae are thought to offer great potential as expression system for various industrial, therapeutic and diagnostic recombinant proteins as they combine high growth rates with all benefits of eukaryotic expression systems. Moreover, microalgae exhibit a phototrophic lifestyle like land plants, hence protein expression is fuelled by photosynthesis, which is CO2-neutral and involves only low production costs. So far, however, research on algal bioreactors for recombinant protein expression is very rare calling for further investigations in this highly promising field. In this study, we present data on the expression of a monoclonal human IgG antibody against the Hepatitis B surface protein and the respective antigen in the diatom Phaeodactylum tricornutum. Antibodies are fully-assembled and functional and accumulate to 8.7% of total soluble protein, which complies with 21 mg antibody per gram algal dry weight. The Hepatitis B surface protein is functional as well and is recognized by algae-produced and commercial antibodies.
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