Essential elements of radical pair magnetosensitivity in Drosophila.
Essential elements of radical pair magnetosensitivity in Drosophila.
复制标题
DOI：
10.1038/s41586-023-05735-z
复制
发表时间：
2023-03
期刊：
影响因子：
64.8
通讯作者：
Baines, Richard A.
中科院分区：
文献类型：
--
作者：
Bradlaugh, Adam A.;Fedele, Giorgio;Munro, Anna L.;Hansen, Celia Napier;Hares, John M.;Patel, Sanjai;Kyriacou, Charalambos P.;Jones, Alex R.;Rosato, Ezio;Baines, Richard A.
许多动物利用地球的磁场（也称为地磁场）进行导航。磁敏感性的有利机制涉及黄素腺嘌呤二核苷酸（FAD）和光感受器蛋白CRY内的色氨酸残基链之间的蓝光激活的电子转移反应。由此产生的自由基对的自旋状态，以及处于其活跃状态的CRY的浓度，受到地磁场的影响。然而，经典的以神经元为中心的自由基对机制并不能解释许多生理和行为观察。在这里，使用电生理学和行为分析，我们在单个神经元和生物体水平上测定磁场反应。我们发现，果蝇CRY的52个C-末端氨基酸残基，缺乏典型的FAD-结合域和色氨酸链，足以促进磁感受。我们还表明，增加细胞内FAD增强蓝光诱导和磁场依赖的C端介导的活性的影响。高水平的FAD单独足以引起蓝光神经元的敏感性，特别是，在磁场的共同存在下，这种反应的增强。这些结果揭示了果蝇初级磁感受器的基本组成部分，提供了强有力的证据表明，非经典（即非CRY依赖）自由基对可以引起细胞的磁场反应。非光敏色素依赖的自由基对可引起果蝇神经元对磁场的反应。
Many animals use Earth’s magnetic field (also known as the geomagnetic field) for navigation. The favoured mechanism for magnetosensitivity involves a blue-light-activated electron-transfer reaction between flavin adenine dinucleotide (FAD) and a chain of tryptophan residues within the photoreceptor protein CRYPTOCHROME (CRY). The spin-state of the resultant radical pair, and therefore the concentration of CRY in its active state, is influenced by the geomagnetic field. However, the canonical CRY-centric radical-pair mechanism does not explain many physiological and behavioural observations. Here, using electrophysiology and behavioural analyses, we assay magnetic-field responses at the single-neuron and organismal levels. We show that the 52 C-terminal amino acid residues of Drosophila melanogaster CRY, lacking the canonical FAD-binding domain and tryptophan chain, are sufficient to facilitate magnetoreception. We also show that increasing intracellular FAD potentiates both blue-light-induced and magnetic-field-dependent effects on the activity mediated by the C terminus. High levels of FAD alone are sufficient to cause blue-light neuronal sensitivity and, notably, the potentiation of this response in the co-presence of a magnetic field. These results reveal the essential components of a primary magnetoreceptor in flies, providing strong evidence that non-canonical (that is, non-CRY-dependent) radical pairs can elicit magnetic-field responses in cells. Non-CRYPTOCHROME-dependent radical pairs can elicit responses to magnetic fields in neurons in Drosophila melanogaster.
登录
查看更多内容
电磁场通过哭泣的依赖性途径破坏了果蝇中的负岩石。
影响因子：
16.6
作者：
Fedele, Giorgio;Green, Edward W.;Rosato, Ezio;Kyriacou, Charalambos P.
通讯作者：
Kyriacou, Charalambos P.
影响因子：
5.7
作者：
Antill, Lewis M.;Woodward, Jonathan R.
通讯作者：
Woodward, Jonathan R.
影响因子：
4.5
作者：
Fedele G;Edwards MD;Bhutani S;Hares JM;Murbach M;Green EW;Dissel S;Hastings MH;Rosato E;Kyriacou CP
通讯作者：
Kyriacou CP
影响因子：
4.8
作者：
Biskup, Till;Paulus, Bernd;Schleicher, Erik
通讯作者：
Schleicher, Erik
影响因子：
16.6
作者：
Foley, Lauren E.;Gegear, Robert J.;Reppert, Steven M.
通讯作者：
Reppert, Steven M.