Resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing MAPK pathway.
Resveratrol, an antioxidant, protects spinal cord injury in rats by suppressing MAPK pathway.
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DOI：
10.1016/j.sjbs.2016.10.019
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发表时间：
2018-03
影响因子：
4.4
通讯作者：
Shao S
中科院分区：
文献类型：
--
作者：
Fu S;Lv R;Wang L;Hou H;Liu H;Shao S
白藜芦醇是一种存在于葡萄、李子和花生等多种植物中的多酚，具有抗氧化、预防心血管疾病和癌症风险等多种医学特性。然而，白藜芦醇对脊髓再灌注损伤的影响还没有研究。本研究旨在探讨白藜芦醇对一氧化氮合酶（iNOS）/p38 MAPK信号通路的影响，探讨白藜芦醇对脊髓损伤的保护作用。采用肾下腹主动脉夹微型动脉瘤夹模型制作脊髓缺血再灌注损伤模型。检测对照组和实验组iNOS和p38 MAPK的表达，以及亚硝酸盐/硝酸盐、丙二醛（MDA）、高级氧化产物（AOPP）、还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的水平。用10 mg/kg白藜芦醇治疗的IRI诱导的大鼠保护脊髓免受缺血损伤，这得到了脊髓组织匀浆中测量的生物学参数的改善的支持。白藜芦醇治疗组血浆NO2-/NO3-水平、iNOS mRNA和蛋白表达及p38 MAPK磷酸化水平均显著降低。此外，IRI产生的自由基减少白藜芦醇治疗增加酶和非酶抗氧化剂的水平，如GSH，SOD和CAT。两者合计，白藜芦醇的管理保护脊髓缺血与潜在的机制，抑制iNOS/p38 MAPK通路的激活和随后的氧化应激的减少，由于IRI引起的损伤。
Resveratrol, a polyphenol found in various plants, including grapes, plums and peanuts has shown various medIRInal properties, including antioxidant, protection of cardiovascular disease and cancer risk. However, the effects of resveratrol on spinal cord reperfusion injury have not been investigated. Hence, the present study was designed to evaluate the effect of resveratrol on nitric oxide synthase (iNOS)/p38MAPK signaling pathway and to elucidate its regulating effect on the protection of spinal cord injury. Spinal cord ischemia–reperfusion injury (IRI) was performed by the infrarenal abdominal aorta with mini aneurysm clip model. The expressions of iNOS and p38MAPK and the levels of biochemical parameters, including nitrite/nitrate, malondialdehyde (MDA), advanced oxidation products (AOPP), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) were measured in control and experimental groups. IRI-induced rats treated with 10 mg/kg resveratrol protected spinal cord from ischemia injury as supported by improved biological parameters measured in spinal cord tissue homogenates. The resveratrol treatment significantly decreased the levels of plasma nitrite/nitrate, iNOS mRNA and protein expressions and phosphorylation of p38MAPK in IRI-induced rats. Further, IRI-produced free radicals were reduced by resveratrol treatment by increasing enzymatic and non-enzymatic antioxidant levels such as GSH, SOD and CAT. Taken together, administration of resveratrol protects the damage caused by spinal cord ischemia with potential mechanism of suppressing the activation of iNOS/p38MAPK pathway and subsequent reduction of oxidative stress due to IRI.
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影响因子：
3.6
作者：
Nalk, Ajit K.;Tandan, Surendra K.;Kumar, Dinesh
通讯作者：
Kumar, Dinesh
影响因子：
4.2
作者：
Maggio, Dominic M.;Chatzipanteli, Katina;Pearse, Damien D.
通讯作者：
Pearse, Damien D.
影响因子：
11.2
作者：
DAWSON, TM;DAWSON, VL;SNYDER, SH
通讯作者：
SNYDER, SH
影响因子：
4.3
作者：
Cambria, RP;Davison, JK;Abbott, WM
通讯作者：
Abbott, WM
DOI：
10.1126/science.1161566
发表时间：
2008-11-07
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Park KK;Liu K;Hu Y;Smith PD;Wang C;Cai B;Xu B;Connolly L;Kramvis I;Sahin M;He Z
通讯作者：
He Z