Fecal Klebsiella pneumoniae Carriage Is Intermittent and of High Clonal Diversity.
Fecal Klebsiella pneumoniae Carriage Is Intermittent and of High Clonal Diversity.
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DOI：
10.3389/fmicb.2020.581081
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发表时间：
2020
影响因子：
5.2
通讯作者：
Ruppitsch W
中科院分区：
文献类型：
--
作者：
Lepuschitz S;Hauser K;Schriebl A;Schlagenhaufen C;Stöger A;Chakeri A;Vötsch K;Pekard-Amenitsch S;Springer B;Allerberger F;Ruppitsch W
肺炎克雷伯菌复合体包括几个密切相关的实体，它们在自然环境中无处不在，包括在植物、动物和人类中。肺炎克雷伯菌是这个复合体中的主要种类。肺炎克雷伯菌菌株是机会性病原体和卫生保健相关感染的常见原因。肺炎克雷伯菌可定植于人类胃肠道，这可能成为感染的储存库。本研究的目的是调查6名健康个体在1年期间的粪便肺炎克雷伯菌携带情况。每周采集一次粪便样本。使用直接和预富集的培养物在补充氨苄西林的琼脂板上划线，每个阳性样本最多选择8个单独的菌落进行进一步表征。采用全基因组测序（WGS）对菌株进行鉴定。从WGS数据中提取序列型（ST）、核心基因组复合体型（CT）、K和O血清型、毒力性状、抗生素耐药性谱和质粒。从6名受试者中5名的278份粪便样本的48份阳性培养物中共分离出80株肺炎克雷伯菌。五名志愿者的样本分别最多产生两种、三种、四种（两人）和五种不同的菌株。80株肺炎克雷伯菌属60株STs，其中9株为新STs；共70个ct，获得48个K血清型，11个O血清型，39个wzc和51个wzi等位基因。5名受试者中有4名携带血清型K20和K47，以及ST37、ST101、ST1265和ST20，这些血清型先前与高风险肺炎克雷伯菌克隆有关。在所有80株菌株中共检测到25个抗生素耐药基因和42个毒力基因。在65株分离株中发现了15种不同类型的质粒。个别菌株携带粪便时间短，仅在一个采样日就发现了70株，连续两周采集的样本中分离到5株。在研究期间，五个被定植的个体中有两个（一起吃饭的同事）四次共享相同的肺炎克雷伯菌类型。我们的发现指出了食物作为人类肺炎克雷伯菌储存库的潜在作用。
The Klebsiella pneumoniae complex comprises several closely related entities, which are ubiquitous in the natural environment, including in plants, animals, and humans. K. pneumoniae is the major species within this complex. K. pneumoniae strains are opportunistic pathogens and a common cause of healthcare-associated infections. K. pneumoniae can colonize the human gastrointestinal tract, which may become a reservoir for infection. The aim of this study was to investigate the fecal K. pneumoniae carriage in six healthy individuals during a 1 year period. Stool samples were obtained once a week. Using direct and pre-enriched cultures streaked on ampicillin-supplemented agar plates, up to eight individual colonies per positive sample were selected for further characterization. Whole genome sequencing (WGS) was performed for strain characterization. Sequence type (ST), core genome complex type (CT), K and O serotypes, virulence traits, antibiotic resistance profiles, and plasmids were extracted from WGS data. In total, 80 K. pneumoniae isolates were obtained from 48 positive cultures of 278 stool samples from five of the six test subjects. The samples of the five colonized volunteers yielded at most two, three, four (two persons), and five different strains, respectively. These 80 K. pneumoniae isolates belonged to 60 STs, including nine new STs; they were of 70 CTs, yielded 48 K serotypes, 11 O serotypes, and 39 wzc and 51 wzi alleles. Four of the five subjects harbored serotypes K20 and K47, as well as STs ST37, ST101, ST1265, and ST20, which had previously been linked to high-risk K. pneumoniae clones. In total, 25 genes conferring antibiotic resistance and 42 virulence genes were detected among all 80 isolates. Plasmids of 15 different types were found among 65 of the isolates. Fecal carriage of individual strains was of short duration: 70 strains were found on a single sampling day only, and 5 strains were isolated in samples collected over two consecutive weeks. Two of the five colonized individuals—working colleagues having meals together—shared identical K. pneumoniae types four times during the study period. Our findings point toward the potential role of food as a reservoir for K. pneumoniae in humans.
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DOI：
10.1093/cid/cix270
发表时间：
2017-07-15
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Gorrie CL;Mirceta M;Wick RR;Edwards DJ;Thomson NR;Strugnell RA;Pratt NF;Garlick JS;Watson KM;Pilcher DV;McGloughlin SA;Spelman DW;Jenney AWJ;Holt KE
通讯作者：
Holt KE
影响因子：
12.2
作者：
Huynh BT;Passet V;Rakotondrasoa A;Diallo T;Kerleguer A;Hennart M;Lauzanne A;Herindrainy P;Seck A;Bercion R;Borand L;Pardos de la Gandara M;Delarocque-Astagneau E;Guillemot D;Vray M;Garin B;Collard JM;Rodrigues C;Brisse S
通讯作者：
Brisse S
影响因子：
4.2
作者：
Collingwood, Abigail;Blostein, Freida;Bachman, Michael A.
通讯作者：
Bachman, Michael A.
影响因子：
2.2
作者：
Lima, Ana Beatriz Mori;Leão, Lara Stefânia Netto de Oliveira;Pimenta, Fabiana Cristina
通讯作者：
Pimenta, Fabiana Cristina
过度抗呼吸和多药的肺炎克隆基团的基因组定义。
影响因子：
11.8
作者：
Bialek-Davenet S;Criscuolo A;Ailloud F;Passet V;Jones L;Delannoy-Vieillard AS;Garin B;Le Hello S;Arlet G;Nicolas-Chanoine MH;Decré D;Brisse S
通讯作者：
Brisse S