In vivo electrophysiological effects of insulin in the rat brain.
In vivo electrophysiological effects of insulin in the rat brain.
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胰岛素对大鼠大脑的体内电生理作用。
DOI：
10.1016/j.npep.2009.05.006
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发表时间：
2009-08
期刊：
影响因子：
2.9
通讯作者：
Hajnal, Andras
中科院分区：
文献类型：
--
作者：
Kovacs, Peter;Hajnal, Andras
脑胰岛素具有广泛的代谢、神经营养和神经调节功能，参与食物摄入和体重、学习和记忆、神经元发育、神经元凋亡和衰老的中枢调节。为了了解胰岛素的神经调节作用，我们旨在表征其尚未定义的体内电生理效应。我们选择从小脑皮质进行记录，因为该区域具有与整个大脑相关的平均胰岛素浓度和胰岛素受体含量，并且之前已被证明是胰岛素信号传导的目标。我们使用体内微离子电渗疗法在神经元旁边应用胰岛素，同时记录自发神经元活动的变化。该分析包括从大鼠 47 个小脑神经元记录的 553 个对胰岛素和其他相关药物的显着神经元反应。我们发现（1）胰岛素刺激对所有记录的神经元产生即时且可逆的电生理效应，并且（2）这些效应主要依赖于先前或同时的 GABA 刺激（94-96%）。具体来说，(a) 对胰岛素的抑制反应是最常见的 (58-62%)，并且与 GABA 预处理呈剂量依赖性，并可通过联合施用胰岛素受体抑制剂 HNMPA 来阻断。 (b) 在第二大神经元群体 (32-38%) 中，共同使用胰岛素时，胰岛素降低了 GABA 抑制的程度。 (c) 相比之下，只有少数神经元对胰岛素应用表现出与 GABA 无关的反应 (4-6%)，这些反应完全是神经元兴奋。目前的研究结果表明，胰岛素对体内中枢神经元具有直接的电生理作用，并且这些作用很大程度上受到 GABA 能输入的影响。
Brain insulin has widespread metabolic, neurotrophic, and neuromodulatory functions and is involved in the central regulation of food intake and body weight, learning and memory, neuronal development, neuronal apoptosis, and aging. To understand the neuromodulatory role of insulin, we aimed to characterize its yet undefined in vivo electrophysiological effects. We elected to record from the cerebellar cortex because as this region has average insulin concentration and insulin receptor content in relation to the whole brain, and has been previously shown to be a target for insulin signaling. We used in vivo microiontophoresis to apply insulin juxtaneuronally while simultaneously recording changes in spontaneous neuronal activity. The analysis included 553 significant neuronal responses to insulin and other related agents recorded from 47 cerebellar neurons of the rat. We found that (1) insulin stimulation produced instant and reversible electrophysiological effects on all of the recorded neurons, and that (2) these effects were mostly dependent on prior or simultaneous GABA-stimulation (94–96%). Specifically, (a) inhibitory responses to insulin were the most common (58–62%), and were dose-dependent with respect to GABA pretreatments and blocked by co-administration of the insulin receptor inhibitor HNMPA. (b) In the second largest neuronal population (32–38%) insulin decreased the magnitude of GABA inhibitions when co-applied. (c) In contrast, only a small number of neurons showed GABA-independent responses to insulin application (4–6%), which were exclusively neuronal excitations. The present findings demonstrate that insulin has direct electrophysiological effects on central neurons in vivo and these effects are highly influenced by GABA-ergic inputs.
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影响因子：
2.6
作者：
Ali, A;Jalees, F;Vohora, D
通讯作者：
Vohora, D
影响因子：
5
作者：
Banks, WA
通讯作者：
Banks, WA
影响因子：
--
作者：
Craft, S;Asthana, S;Veith, RC
通讯作者：
Veith, RC
影响因子：
5.6
作者：
Bonnet, U;Bingmann, D;Wiemann, M
通讯作者：
Wiemann, M
DOI：
10.1073/pnas.90.18.8752
发表时间：
1993-09-15
影响因子：
11.1
作者：
CALISSANO, P;CIOTTI, MT;MERCANTI, D
通讯作者：
MERCANTI, D