Investigation of Pain Mechanisms by Calcium Imaging Approaches.
Investigation of Pain Mechanisms by Calcium Imaging Approaches.
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DOI：
10.1007/s12264-017-0139-9
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发表时间：
2018-03
影响因子：
5.6
通讯作者：
Dong X
中科院分区：
文献类型：
--
作者：
Anderson M;Zheng Q;Dong X
由于涉及躯体感觉的复杂电路和过多的细胞类型，能够在群体水平上观察细胞活动变得越来越重要。此外，由于细胞依赖于各种复杂的细胞外因子，因此努力维持生理环境很重要。许多电生理学技术需要人工产生的生理环境的实施，并且可能难以同时评估许多细胞的活性。此外，使用Ca2+敏感染料成像Ca2+瞬变通常需要体外制备或体内注射，这可能导致可变的表达水平。随着更敏感的遗传编码的Ca2+指示剂（GECIs）的发展，现在可以同时观察大量细胞中Ca2+瞬变的变化。最近，一些研究小组使用一种称为GCaMP的GECI来解决躯体感觉中的基本问题。研究人员现在可以使用病毒或基因敲入方法在小鼠基因组中诱导GCaMP表达，并观察疼痛通路中细胞群的活性，如背根神经节（DRG），脊髓神经元或神经胶质细胞。这种方法可以在体内使用，从而保持生物体的生物完整性。GCaMP成像的实现使我们对躯体感觉的理解取得了许多进展。在这里，我们回顾了目前的研究结果在疼痛研究中使用GCaMP成像以及讨论潜在的方法学考虑。
Due to the complex circuitry and plethora of cell types involved in somatosensation, it is becoming increasingly important to be able to observe cellular activity at the population level. In addition, since cells rely on an intricate variety of extracellular factors, it is important to strive to maintain the physiological environment. Many electrophysiological techniques require the implementation of artificially-produced physiological environments and it can be difficult to assess the activity of many cells simultaneously. Moreover, imaging Ca2+ transients using Ca2+-sensitive dyes often requires in vitro preparations or in vivo injections, which can lead to variable expression levels. With the development of more sensitive genetically-encoded Ca2+ indicators (GECIs) it is now possible to observe changes in Ca2+ transients in large populations of cells at the same time. Recently, groups have used a GECI called GCaMP to address fundamental questions in somatosensation. Researchers can now induce GCaMP expression in the mouse genome using viral or gene knock-in approaches and observe the activity of populations of cells in the pain pathway such as dorsal root ganglia (DRG), spinal neurons, or glia. This approach can be used in vivo and thus maintains the organism’s biological integrity. The implementation of GCaMP imaging has led to many advances in our understanding of somatosensation. Here, we review the current findings in pain research using GCaMP imaging as well as discussing potential methodological considerations.
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影响因子：
16.6
作者：
Sekiguchi KJ;Shekhtmeyster P;Merten K;Arena A;Cook D;Hoffman E;Ngo A;Nimmerjahn A
通讯作者：
Nimmerjahn A
影响因子：
3.7
作者：
Ohkura M;Sasaki T;Sadakari J;Gengyo-Ando K;Kagawa-Nagamura Y;Kobayashi C;Ikegaya Y;Nakai J
通讯作者：
Nakai J
影响因子：
48
作者：
Farrar, Matthew J.;Bernstein, Ida M.;Schlafer, Donald H.;Cleland, Thomas A.;Fetcho, Joseph R.;Schaffer, Chris B.
通讯作者：
Schaffer, Chris B.
DOI：
10.1073/pnas.0509378103
发表时间：
2006-03-21
影响因子：
11.1
作者：
Tallini, YN;Ohkura, M;Kotlikoff, MI
通讯作者：
Kotlikoff, MI
影响因子：
5.5
作者：
Schmidt, H;Stiefel, KM;Eilers, J
通讯作者：
Eilers, J