Drug Resistant Breast Cancer Cell Line Displays Cancer Stem Cell Phenotype and Responds Sensitively to Epigenetic Drug SAHA.
Drug Resistant Breast Cancer Cell Line Displays Cancer Stem Cell Phenotype and Responds Sensitively to Epigenetic Drug SAHA.
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DOI：
10.1007/s13346-012-0113-z
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发表时间：
2013-04-01
影响因子：
5.4
通讯作者：
Labhasetwar, Vinod
中科院分区：
文献类型：
--
作者：
Lu, Shan;Labhasetwar, Vinod
关键词：
人类乳腺实体肿瘤中的癌症干细胞（CSC）群体通过CD44+/CD24−表型进行鉴定，其特点是高致瘤性、侵袭性和耐药性。在本研究中，我们利用流式细胞术、免疫荧光、微球形成实验和迁移实验对耐药乳腺癌细胞系MCF-7/Adr和一些乳腺癌细胞系进行了表征，分别检查了它们的CSC免疫表型、CSC蛋白的存在、体外致瘤性和迁移率。我们的结果表明，MCF-7/Adr 细胞均匀地表现出 CSC 特征，但保留较低的迁移率。它们还能够在漂浮培养条件下自我更新和分化。此外，MCF-7/Adr 对表观遗传药物辛二酰苯胺异羟肟酸 (SAHA) 选择性敏感，失去耐药性并改变形态，但保留 CSC 免疫表型。总之，我们表明耐药乳腺癌细胞系 MCF-7/Adr 表现出一致的 CSC 样特征，并且对表观遗传药物治疗敏感。
Cancer stem cell (CSC) population in solid human breast tumor was identified by CD44+/CD24− phenotype, characterized by high tumorigenicity, invasiveness and drug resistance. In this study, we characterized drug resistant breast cancer cell line-MCF-7/Adr and a number of breast cancer cell lines using flow cytometry, immunofluorescence, mammosphere formation assay and migration assay, examining their CSC immunophenotypes, presence of CSC proteins, tumorigenicity in vitro and migratory rates, respectively. Our results show that MCF-7/Adr cells uniformly display CSC characteristics yet retain low migratory rate. They are also able to self-renew and differentiate under floating culture conditions. Furthermore, MCF-7/Adr is selectively sensitive to epigenetic drug, suberoylanilide hydroxamic acid (SAHA), losing drug resistance and changes morphology yet retaining CSC immunophenotypes. In conclusion, we show that resistant breast cancer cell line MCF-7/Adr demonstrates uniform CSC like characteristics and are sensitive to epigenetic drug treatment.
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影响因子：
7.4
作者：
Fillmore, Christine M.;Kuperwasser, Charlotte
通讯作者：
Kuperwasser, Charlotte
影响因子：
7.4
作者：
Sheridan, Carol;Kishimoto, Hiromitsu;Fuchs, Robyn K.;Mehrotra, Sanjana;Bhat-Nakshatri, Poornima;Turner, Charles H.;Goulet, Robert, Jr.;Badve, Sunil;Nakshatri, Harikrishna
通讯作者：
Nakshatri, Harikrishna
影响因子：
30.8
作者：
Bass, Adam J.;Watanabe, Hideo;Mermel, Craig H.;Yu, Soyoung;Perner, Sven;Verhaak, Roel G.;Kim, So Young;Wardwell, Leslie;Tamayo, Pablo;Gat-Viks, Irit;Ramos, Alex H.;Woo, Michele S.;Weir, Barbara A.;Getz, Gad;Beroukhim, Rameen;O'Kelly, Michael;Dutt, Amit;Rozenblatt-Rosen, Orit;Dziunycz, Piotr;Komisarof, Justin;Chirieac, Lucian R.;LaFargue, Christopher J.;Scheble, Veit;Wilbertz, Theresia;Ma, Changqing;Rao, Shilpa;Nakagawa, Hiroshi;Stairs, Douglas B.;Lin, Lin;Giordano, Thomas J.;Wagner, Patrick;Minna, John D.;Gazdar, Adi F.;Zhu, Chang Qi;Brose, Marcia S.;Cecconello, Ivan;Ribeiro, Ulysses, Jr.;Marie, Suely K.;Dahl, Olav;Shivdasani, Ramesh A.;Tsao, Ming-Sound;Rubin, Mark A.;Wong, Kwok K.;Regev, Aviv;Hahn, William C.;Beer, David G.;Rustgi, Anil K.;Meyerson, Matthew
通讯作者：
Meyerson, Matthew
影响因子：
11.2
作者：
Baumann, P;Cremers, N;Sleeman, JP
通讯作者：
Sleeman, JP
DOI：
10.1186/bcr2111
发表时间：
2008
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Morrison BJ;Schmidt CW;Lakhani SR;Reynolds BA;Lopez JA
通讯作者：
Lopez JA