Analyzing the Roles of Rho GTPases in Cancer Cell Adhesion to Endothelial Cells Under Flow Conditions.

Analyzing the Roles of Rho GTPases in Cancer Cell Adhesion to Endothelial Cells Under Flow Conditions.
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分析流动条件下 Rho GTP 酶在癌细胞粘附内皮细胞中的作用。

DOI:
10.1007/978-1-0716-1350-4_7
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发表时间:
2021
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Cerutti C
Cerutti C
中科院分区:
--
文献类型:
--
作者:
Cerutti C

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癌细胞与血管内皮细胞之间的粘附是肿瘤进展和转移形成中的重要事件。Rho GTP酶的表达在癌症中经常改变,并且已知它们通过其对粘附和细胞骨架动力学的影响来调节细胞迁移。几种不同类型的测定用于研究癌细胞如何附着和穿过内皮。在这里,我们描述了一种体外技术来研究在剪切应力耦合到活细胞成像下Rho GTPases对人癌细胞粘附到内皮细胞的影响。
Adhesion between cancer cells and endothelial cells, lining the blood vessels, is an important event in tumor progression and metastasis formation. The expression of Rho GTPases is frequently altered in cancers, and they are known to regulate cell migration through their effects on adhesion and cytoskeletal dynamics. Several different types of assays are used to investigate how cancer cells attach to and cross the endothelium. Here, we describe an in vitro technique to study the effects of Rho GTPases on human cancer cell adhesion to endothelial cells under shear stress coupled to live cell imaging.
DOI: 10.1038/srep45284
发表时间: 2017-03-30
期刊: Scientific reports
影响因子: 4.6
作者:
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通讯作者: Romero IA
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DOI: --
发表时间: 2020
期刊: Troubleshooting Centrifugal Pumps and their Systems
影响因子: --
作者:
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发表时间: 2017-10-15
期刊: Cancer research
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DOI: --
发表时间: 2012
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