Microneedle Vaccination Elicits Superior Protection and Antibody Response over Intranasal Vaccination against Swine-Origin Influenza A (H1N1) in Mice.
Microneedle Vaccination Elicits Superior Protection and Antibody Response over Intranasal Vaccination against Swine-Origin Influenza A (H1N1) in Mice.
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DOI：
10.1371/journal.pone.0130684
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kim YC
中科院分区：
文献类型：
--
作者：
Shin JH;Park JK;Lee DH;Quan FS;Song CS;Kim YC
流感是全球重大传染病之一，疫苗接种被认为是预防的最佳途径。本研究比较了猪源性甲型H1N1流感病毒灭活疫苗鼻内（In）和微针（MN）两种疫苗的免疫原性和保护效果。小鼠分别用1 μg H1N1病毒灭活疫苗给药MN或IN。免疫后测定所有免疫血清抗原特异性抗体反应和血凝抑制（HI）滴度。免疫后5周，进行致死攻击以评估保护效果。此外，小鼠以更高剂量（> 1 μg）接种IN，以相同的方法进行分析，并与1 μg疫苗包被MN进行比较。MN组血清抗原特异性抗体反应和HI滴度明显高于in组。MN组的保护率为100%，体重轻微减轻，病毒复制减少，而in组的存活率为0%。随着免疫球蛋白剂量的增加，mn免疫血清的抗原特异性抗体反应和HI滴度明显高于其他免疫球蛋白组。此外，1 μg-MN组的保护性免疫与20、40 μg-IN组相似。我们得出结论，在相同的疫苗剂量下，MN疫苗接种比IN疫苗接种具有更大的潜在免疫应答和保护作用。
Influenza is one of the critical infectious diseases globally and vaccination has been considered as the best way to prevent. In this study, immunogenicity and protection efficacy between intranasal (IN) and microneedle (MN) vaccination was compared using inactivated swine-origin influenza A/H1N1 virus vaccine. Mice were vaccinated by MN or IN administration with 1 μg of inactivated H1N1 virus vaccine. Antigen-specific antibody responses and hemagglutination-inhibition (HI) titers were measured in all immunized sera after immunization. Five weeks after an immunization, a lethal challenge was performed to evaluate the protective efficacy. Furthermore, mice were vaccinated by IN administration with higher dosages (> 1 μg), analyzed in the same manner, and compared with 1 μg-vaccine-coated MN. Significantly higher antigen-specific antibody responses and HI titer were measured in sera in MN group than those in IN group. While 100% protection, slight weight loss, and reduced viral replication were observed in MN group, 0% survival rate were observed in IN group. As vaccine dose for IN vaccination increased, MN-immunized sera showed much higher antigen-specific antibody responses and HI titer than other IN groups. In addition, protective immunity of 1 μg-MN group was similar to those of 20- and 40 μg-IN groups. We conclude that MN vaccination showed more potential immune response and protection than IN vaccination at the same vaccine dosage.
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DOI：
10.1086/649228
发表时间：
2010-01-15
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Kim YC;Quan FS;Yoo DG;Compans RW;Kang SM;Prausnitz MR
通讯作者：
Prausnitz MR
影响因子：
6.4
作者：
Ichinohe, Takeshi;Tamura, Shin-ichi;Hasegawa, Hideki
通讯作者：
Hasegawa, Hideki
影响因子：
5.5
作者：
Girard MP;Cherian T;Pervikov Y;Kieny MP
通讯作者：
Kieny MP
影响因子：
3.3
作者：
Chen, Xi;Zhi, Feng;Yang, Yilin
通讯作者：
Yang, Yilin
影响因子：
5.5
作者：
Buonagurio, DA;Bechert, TM;Udem, SA
通讯作者：
Udem, SA