Microarray karyotyping of maltose-fermenting Saccharomyces yeasts with differing maltotriose utilization profiles reveals copy number variation in genes involved in maltose and maltotriose utilization.
Microarray karyotyping of maltose-fermenting Saccharomyces yeasts with differing maltotriose utilization profiles reveals copy number variation in genes involved in maltose and maltotriose utilization.
复制标题
麦芽糖发酵糖脂酵母的微阵列核分型具有不同的麦芽三糖利用率剖面揭示了与麦芽糖和麦芽糖利用相关的基因的拷贝数变化。
DOI：
10.1111/j.1365-2672.2009.04656.x
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发表时间：
2010-07
影响因子：
4
通讯作者：
Stambuk BU
中科院分区：
文献类型：
--
作者：
Duval EH;Alves SL Jr;Dunn B;Sherlock G;Stambuk BU
我们对 52 种能够有效发酵麦芽糖的酵母菌株的麦芽三糖利用进行了分析，并将观察到的表型与可能参与酵母细胞麦芽三糖利用的基因拷贝数差异相关联。使用微型液体培养以及需氧分批培养，对酿酒酵母和巴氏酵母（天然酿酒酵母/巴彦酵母杂交种）的实验室和工业菌株的麦芽糖和麦芽三糖利用进行了分析。所有菌株均有效利用麦芽糖作为碳源，但观察到麦芽三糖利用的三种不同表型：高效生长、缓慢/延迟生长和不生长。通过微阵列核型分析和脉冲场凝胶电泳印迹，我们分析了选定的酿酒酵母菌株中几个麦芽糖相关基因的拷贝数和定位。虽然大多数菌株缺乏 MPH2 和 MPH3 转运蛋白基因，但几乎所有分析的菌株都具有 AGT1 基因和增加的 MALx1 通透酶拷贝数。我们的结果表明，巴斯德酵母菌株比酿酒酵母菌株更有效地利用麦芽三糖，并强调了 AGT1 基因对于酿酒酵母有效利用麦芽三糖的重要性。我们的结果揭示了新的麦芽三糖利用表型，有助于更好地了解这种碳源的代谢，以改善酵母菌的发酵。
We performed an analysis of maltotriose utilization by 52 Saccharomyces yeast strains able to ferment maltose efficiently and correlated the observed phenotypes with differences in the copy number of genes possibly involved in maltotriose utilization by yeast cells. The analysis of maltose and maltotriose utilization by laboratory and industrial strains of the species Saccharomyces cerevisiae and Saccharomyces pastorianus (a natural S. cerevisiae/Saccharomyces bayanus hybrid) was carried out using microscale liquid cultivation, as well as in aerobic batch cultures. All strains utilize maltose efficiently as a carbon source, but three different phenotypes were observed for maltotriose utilization: efficient growth, slow/delayed growth and no growth. Through microarray karyotyping and pulsed-field gel electrophoresis blots, we analysed the copy number and localization of several maltose-related genes in selected S. cerevisiae strains. While most strains lacked the MPH2 and MPH3 transporter genes, almost all strains analysed had the AGT1 gene and increased copy number of MALx1 permeases. Our results showed that S. pastorianus yeast strains utilized maltotriose more efficiently than S. cerevisiae strains and highlighted the importance of the AGT1 gene for efficient maltotriose utilization by S. cerevisiae yeasts. Our results revealed new maltotriose utilization phenotypes, contributing to a better understanding of the metabolism of this carbon source for improved fermentation by Saccharomyces yeasts.
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影响因子：
2.4
作者：
Crumplen, RM;Slaughter, JC;Stewart, GG
通讯作者：
Stewart, GG
影响因子：
2.7
作者：
Londesborough, J
通讯作者：
Londesborough, J
影响因子：
1.2
作者：
Batista, AS;Miletti, LC;Stambuk, BU
通讯作者：
Stambuk, BU
影响因子：
4.1
作者：
Brito dos Santos, Scheila Karina;Basilio, Anna Carla Moreira;de Morais, Marcos, Jr.
通讯作者：
de Morais, Marcos, Jr.
影响因子：
2.6
作者：
Meneses, FJ;Jiranek, V
通讯作者：
Jiranek, V