The three major types of CRISPR-Cas systems function independently in CRISPR RNA biogenesis in Streptococcus thermophilus.
The three major types of CRISPR-Cas systems function independently in CRISPR RNA biogenesis in Streptococcus thermophilus.
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DOI：
10.1111/mmi.12644
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发表时间：
2014-07
影响因子：
3.6
通讯作者：
Terns MP
中科院分区：
文献类型：
--
作者：
Carte J;Christopher RT;Smith JT;Olson S;Barrangou R;Moineau S;Glover CV 3rd;Graveley BR;Terns RM;Terns MP
CRISPR-CAS系统是基于RNA的小免疫系统，保护原核生物免受病毒和质粒等入侵者的侵袭。我们研究了嗜热链球菌(Sth)菌株DGCC7710中CRISPR(Cr)RNA的特征和生物发生，该菌株拥有四种不同的CRISPR-Cas系统，其中包括三种主要类型的CRISPR-Cas系统的代表。我们的结果表明，来自每个CRISPR基因座的crRNAs被与各自基因座相关的Cas蛋白(和非编码RNA)特异性地加工成不同的crRNA物种。我们发现CSM III-A型和CSE I-E型crRNAs分别被Cas6和Cse3(Cas6e)特异性处理，并保留了入侵者靶向序列的8核苷酸CRISPR重复序列标签5‘。CSE类型I-E crRNAs还保留21个核苷酸的3‘重复标签。来自Sth中的两个CSN II-A型系统的crRNA由5‘-截短的靶向序列和3’标签组成；然而，它们在大小上是不同的。此外，与一个CSN基因座相关的CSN1(Cas9)蛋白在从该基因座产生crRNAs的过程中起着特殊的作用。我们的发现表明，多个CRISPR-CAS系统可以在给定生物体内的crRNA生物发生中独立发挥作用-这是设计共存的CRISPR-CAS途径的一个重要考虑因素。
CRISPR-Cas systems are small RNA-based immune systems that protect prokaryotes from invaders such as viruses and plasmids. We have investigated the features and biogenesis of the CRISPR (cr)RNAs in Streptococcus thermophilus (Sth) strain DGCC7710, which possesses four different CRISPR-Cas systems including representatives from the three major types of CRISPR-Cas systems. Our results indicate that the crRNAs from each CRISPR locus are specifically processed into divergent crRNA species by Cas proteins (and non-coding RNAs) associated with the respective locus. We find that the Csm Type III-A and Cse Type I-E crRNAs are specifically processed by Cas6 and Cse3 (Cas6e), respectively, and retain an 8-nucleotide CRISPR repeat sequence tag 5′ of the invader-targeting sequence. The Cse Type I-E crRNAs also retain a 21-nucleotide 3′ repeat tag. The crRNAs from the two Csn Type II-A systems in Sth consist of a 5′-truncated targeting sequence and a 3′ tag; however these are distinct in size between the two. Moreover, the Csn1 (Cas9) protein associated with one Csn locus functions specifically in the production of crRNAs from that locus. Our findings indicate that multiple CRISPR-Cas systems can function independently in crRNA biogenesis within a given organism – an important consideration in engineering co-existing CRISPR-Cas pathways.
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影响因子：
16
作者：
Hale, Caryn R.;Majumdar, Sonali;Elmore, Joshua;Pfister, Neil;Compton, Mark;Olson, Sara;Resch, Alissa M.;Glover, Claiborne V. C., III;Graveley, Brenton R.;Terns, Rebecca M.;Terns, Michael P.
通讯作者：
Terns, Michael P.
影响因子：
3.2
作者：
Deveau, Helene;Barrangou, Rodolphe;Moineau, Sylvain
通讯作者：
Moineau, Sylvain
影响因子：
4.5
作者：
Hale, Caryn;Kleppe, Kyle;Terns, Michael P.
通讯作者：
Terns, Michael P.
影响因子：
4.4
作者：
HILL, C;MASSEY, IJ;KLAENHAMMER, TR
通讯作者：
KLAENHAMMER, TR
影响因子：
46.9
作者：
Cho, Seung Woo;Kim, Sojung;Kim, Jin-Soo
通讯作者：
Kim, Jin-Soo