Expression of transient receptor potential (TRP) channel mRNAs in the mouse olfactory bulb.
Expression of transient receptor potential (TRP) channel mRNAs in the mouse olfactory bulb.
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DOI：
10.1016/j.neulet.2012.07.013
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发表时间：
2012-08-22
影响因子：
2.5
通讯作者：
Ennis M
中科院分区：
文献类型：
--
作者：
Dong HW;Davis JC;Ding S;Nai Q;Zhou FM;Ennis M
瞬时受体电位(Trp)通道是一大类阳离子通道。啮齿动物的28个Trp通道亚型分为6个亚家族：TRPC1-7、TRPV1-6、TRPM1-8、TRPP2/3/5、TRPML1-3和TRPA1。色氨酸通道参与外周嗅觉传导。几个TRPC通道在主嗅球(OB)的不明神经元中表达，但大多数Trp通道在OB中的表达尚未被研究。本研究采用RT-PCR方法初步研究了通道基因在小鼠卵圆细胞和3种细胞类型中的表达：体外簇生细胞、二尖瓣细胞和颗粒细胞。除TRPV5外，其余TRP通道基因均在OB组织中表达。单细胞RT-PCR结果显示，体外簇状、二尖瓣和颗粒细胞群共表达14个Trp通道mRNAs，涵盖所有6个亚家族的成员。这些不同的OB神经元群体表达7-12个通道的mRNAs。共同通道的表达在外部簇状细胞和二尖瓣细胞之间比在这些细胞和颗粒细胞之间更相似。这些结果表明，OB神经元表达了大量的Trp通道亚型，为这些通道调节OB神经元的活动和中枢嗅觉处理提供了分子基础。
Transient receptor potential (TRP) channels are a large family of cation channels. The 28 TRP channel subtypes in rodent are divided into 6 subfamilies: TRPC1-7, TRPV1-6, TRPM1-8, TRPP2/3/5, TRPML1-3 and TRPA1. TRP channels are involved in peripheral olfactory transduction. Several TRPC channels are expressed in unidentified neurons in the main olfactory bulb (OB), but the expression of most TRP channels in the OB has not been investigated. The present study employed RT-PCR as an initial survey of the expression of channel mRNAs in the mouse OB and in 3 cell types: external tufted, mitral and granule cells. All TRP channel mRNAs except TRPV5 were detected in OB tissue. Single cell RT-PCR revealed that external tufted, mitral and granule cell populations expressed in aggregate 14 TRP channel mRNAs encompassing members of all 6 subfamilies. These different OB neuron populations expressed 7 to 12 channel mRNAs. Common channel expression was more similar among external tufted and mitral cells than among these cells and granule cells. These results indicate that a large number of TRP channel subtypes are expressed in OB neurons, providing the molecular bases for these channels to regulate OB neuron activity and central olfactory processing.
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影响因子：
2.5
作者：
Heinbockel, Thomas;Laaris, Nora;Ennis, Matthew
通讯作者：
Ennis, Matthew
DOI：
10.1073/pnas.96.10.5791
发表时间：
1999-05-11
影响因子：
11.1
作者：
Liman, ER;Corey, DP;Dulac, C
通讯作者：
Dulac, C
DOI：
10.1523/jneurosci.4816-09.2010
发表时间：
2010-01-27
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Qiu J;Fang Y;Rønnekleiv OK;Kelly MJ
通讯作者：
Kelly MJ
影响因子：
5.5
作者：
Gee, CE;Benquet, P;Gerber, U
通讯作者：
Gerber, U
影响因子：
1.4
作者：
Nakashimo Y;Takumida M;Fukuiri T;Anniko M;Hirakawa K
通讯作者：
Hirakawa K