Shiga-like-toxin-producing Escherichia coli in retail meats and cattle in Thailand
Shiga-like-toxin-producing Escherichia coli in retail meats and cattle in Thailand
复制标题
泰国零售肉类和牛体内产生志贺样毒素的大肠杆菌
DOI：
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发表时间：
1990
影响因子：
4.4
通讯作者：
P. Echeverria
中科院分区：
文献类型：
--
作者：
O. Suthienkul;J. Brown;J. Seriwatana;S. Tienthongdee;S. Sastravaha;P. Echeverria
特异性DNA探针用于鉴定泰国蔬菜、肉类、牛和农场动物中产生志贺样毒素I （SLT I）和SLT ii的大肠杆菌。从9%的市场牛肉标本、8%至28%的屠宰场新鲜牛肉标本和11%至84%的牛粪便标本中分离出产生slt的大肠杆菌。动物经常感染几种不同的产生SLT的大肠杆菌，这些大肠杆菌与SLT I、SLT II或两种SLT探针杂交。在119株产生SLT的大肠杆菌中，24%与SLT I探针杂交，31%与SLT II探针杂交，44%与两种SLT探针杂交。肠出血性大肠杆菌质粒探针与来自食品和牛的64%（106株中68株）产生slt的大肠杆菌分离株杂交，与来自猪的8%（201株中17株）的大肠杆菌分离株杂交。猪体内未检出产slt的大肠杆菌。与SLT II探针杂交的大肠杆菌分离株中有76%（34个中的26个）对Vero细胞有细胞毒性，但对HeLa细胞没有，这表明它们产生了SLT II的变体。产牛肉动物中产生slt的大肠杆菌的高流行率表明，在泰国，接触动物和食用牛肉可能对获得肠出血性大肠杆菌感染构成健康风险。
Specific DNA probes were used to identify Shiga-like toxin I (SLT I)- and SLT II-producing Escherichia coli in vegetables, meats, cattle, and farm animals in Thailand. SLT-producing E. coli was isolated from 9% of market beef specimens, from 8 to 28% of fresh beef specimens at slaughterhouses, and from 11 to 84% of fecal specimens from cattle. Animals were frequently infected with several different SLT-producing E. coli types that hybridized with either the SLT I, SLT II, or both SLT probes. Of 119 SLT-producing E. coli isolates, 24% hybridized with the SLT I probe, 31% hybridized with the SLT II probe, and 44% hybridized with both SLT probes. The enterohemorrhagic E. coli plasmid probe hybridized with 64% (68 of 106) of SLT-producing E. coli isolates from food and cattle and with 8% (17 of 201) of E. coli isolates from pigs. No SLT-producing E. coli was detected in pigs. Seventy-six percent (26 of 34) of E. coli isolates that hybridized with the SLT II probe were cytotoxic to Vero but not to HeLa cells, suggesting that they produced the variant of SLT II. The high prevalence of SLT-producing E. coli in beef-producing animals suggests that exposure to animals and eating beef may pose a health risk for acquiring enterohemorrhagic E. coli infections in Thailand.
用于识别 O157:H7 肠出血性大肠杆菌和导致出血性结肠炎和溶血性尿毒症综合征的其他血清型的 DNA 探针。
DOI：
10.1093/infdis/156.1.175
发表时间：
1987
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Levine,MM;Xu,JG;Kaper,JB;Lior,H;Prado,V;Tall,B;Nataro,J;Karch,H;Wachsmuth,K
通讯作者：
Wachsmuth,K