Cloning and characterization of the gene encoding the bovine BOULE protein
Cloning and characterization of the gene encoding the bovine BOULE protein
复制标题
牛 BOULE 蛋白编码基因的克隆和表征
DOI：
10.1007/s00438-008-0394-6
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发表时间：
2008
影响因子：
3.1
通讯作者：
中科院分区：
文献类型：
--
作者：
无精子症（DAZ）中缺失的基因编码潜在的rna结合蛋白，这些蛋白只在种系中表达。牛无精子样基因缺失是雄性牛-牦牛不育的有力候选基因。为了进一步揭示雄性牛-牦牛不育的遗传原因，本研究对牛edazfamily的另一个基因b-boule进行了分离和鉴定。预计b-boulegene编码295个氨基酸的多肽，具有rnp型RNA识别结构域。三级结构分析表明，b-boule与多嘧啶rna特异性结合，可能在生殖细胞形成和生殖细胞形态变化过程中发挥核核糖-核蛋白颗粒辅助因子的作用。RT-PCR检测显示b-boule在成年睾丸中特异性表达。然而，在不育雄性牛-牦牛的睾丸中检测到极低的表达水平。不育雄鼠睾丸的显微结构显示，A型精原细胞是唯一存在的生殖细胞，很少有生殖细胞在粗线精母细胞阶段进一步发育。这些结果表明，b- boule1可能在牛精子发生中起作用，低水平的b- boule1表达可能导致牛-牦牛雄性不育。
TheDeleted in Azoospermia(DAZ) genes encode potential RNA-binding proteins that are expressed exclusively in the germ-line. The bovineDeleted in Azoospermia-like gene is a strong candidate for male cattle-yak infertility. In this work, with the goe goal to further reveal the genetic cause of male cattle-yak sterility, another bovineDAZfamily gene, b-boule, was isolated and characterized. The b-boulegene is predicted to encode a polypeptide of 295 amino acids with an RNP-type RNA recognition domain. Tertiary structure analysis shows that b-boule binds specifically to polypyrimidine RNAs and might act as a nuclear ribonucleoprotein particle auxiliary factor during germ cell formation and morphological changes of germ cells. RT-PCR assays revealed that b-boulewas expressed specifically in the adult testis. However, an extremely low level of expression was detected in the testis of sterile male cattle-yaks. Microstructure of the testes from sterile males showed that type A spermatogonia were the only germ cells present and that few germ cells developed further than the stage of pachytene spermatocytes. These results suggest that b-boulemay function in bovine spermatogenesis, and that low levels of b-bouleexpression might lead to male sterility in cattle-yaks.
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影响因子：
5.6
作者：
KROGH, A;BROWN, M;HAUSSLER, D
通讯作者：
HAUSSLER, D
DOI：
10.1093/bioinformatics/12.2.95
发表时间：
1996-04
期刊：
Computer applications in the biosciences : CABIOS
影响因子：
--
作者：
R. Hughey;A. Krogh
通讯作者：
R. Hughey;A. Krogh
影响因子：
64.8
作者：
SCHERLY, D;BOELENS, W;MATTAJ, IW
通讯作者：
MATTAJ, IW
DOI：
--
发表时间：
2001
期刊：
--
影响因子：
--
作者：
Zhang Xujing
通讯作者：
Zhang Xujing
影响因子：
5.8
作者：
Karplus, K;Barrett, C;Hughey, R
通讯作者：
Hughey, R