Exposure of Sperm Head Equatorin after Acrosome Reaction and Its Fate after Fertilization in Mice1
Exposure of Sperm Head Equatorin after Acrosome Reaction and Its Fate after Fertilization in Mice1
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小鼠顶体反应后精子头部赤道素的暴露及其受精后的命运1
DOI：
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发表时间：
2001
影响因子：
3.6
通讯作者：
K. Toshimori
中科院分区：
文献类型：
--
作者：
G. Manandhar;K. Toshimori
摘要赤道素是一种精子头部赤道蛋白，可能参与精子-卵母细胞融合(Toshimori等人，Biol Reprod 1998；59：22-29)。在目前的工作中，我们已经证明，只有在固定和通透后发生自发或诱导的顶体反应后，才能检测到后顶体中包含的赤道素，但在完整的精子中不能检测到。在超声或离子载体处理过程中存在的蛋白水解酶抑制剂不会抑制抗原表位的暴露。位于卵周隙的穿透透明带的精子表现为赤道素，与顶体反应的精子相似。在体外受精(IVF)过程中，精子与卵子融合后，赤道素从精子头赤道区域解离，并留在解聚的男性原核附近。在原核对位阶段，它可能被核周微管推离原核。在第一次卵裂后，原胚胎细胞之一继承了赤道素。在第二次卵裂后，残留的赤道素消失。将整个精子或精子头显微注射到MII期的卵母细胞中，显示出与卵黄周精子类似的赤道素。激活后，它会以类似于试管受精过程中的方式从精子核分离。在受精过程中，赤道素的退化模式与精子尾部成分或线粒体相似，但不同于膜相关蛋白。
Abstract Equatorin is a sperm head equatorial protein, possibly involved in sperm-oocyte fusion (Toshimori et al., Biol Reprod 1998; 59:22–29). In the present work, we have shown that equatorin contained in the posterior acrosome is detectable only after spontaneous or induced acrosome reactions following fixation and permeabilization, but not in intact spermatozoa. The presence of protease inhibitors during sonication or ionophore treatments does not inhibit the exposure of the antigenic epitope. The zona-penetrated spermatozoa lying in the perivitelline space display equatorin, similar to those of the acrosome-reacted ones. After sperm-egg fusion during in vitro fertilization (IVF), the equatorin dissociates from the sperm head equatorial region and remains at the vicinity of the decondensing male pronuclei. During pronuclear apposition stage, it is pushed away from the pronuclei, possibly by the perinuclear microtubules. After first cleavage, equatorin is inherited by one of the proembryonic cells. The residual equatorin disappears after the second cleavage. Microinjected whole spermatozoa or sperm heads into the MII stage oocytes display equatorin similar to those of the perivitelline sperm. After activation, it dissociates from the sperm nuclei in a similar manner as during IVF. The mode of equatorin degeneration during fertilization is similar to those of the sperm tail components or mitochondria, but different from those of the membrane associated proteins.
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受精过程中精子核周卵泡膜的去除及其与牛卵母细胞表面的关联。
DOI：
10.1006/dbio.1997.8618
发表时间：
1997
期刊：
Developmental biology.
影响因子：
--
作者：
Sutovsky,P;Oko,R;Hewitson,L;Schatten,G
通讯作者：
Schatten,G
影响因子：
3.6
作者：
Sutovsky, P;Navara, CS;Schatten, G
通讯作者：
Schatten, G
影响因子：
3.6
作者：
Kuretake, S;Kimura, Y;Yanagimachi, R
通讯作者：
Yanagimachi, R
影响因子：
3.6
作者：
Saling,PM;Irons,G;Waibel,R
通讯作者：
Waibel,R
DOI：
10.1530/jrf.0.0860679
发表时间：
1989-07-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
CHATOT, CL;ZIOMEK, CA;TORRES, I
通讯作者：
TORRES, I