Nanomaterial based self-referencing microbiosensors for cell and tissue physiology research.
Nanomaterial based self-referencing microbiosensors for cell and tissue physiology research.
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DOI：
10.1016/j.bios.2012.06.059
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发表时间：
2013-02-15
影响因子：
12.6
通讯作者：
Porterfield, D. Marshall
中科院分区：
文献类型：
--
作者：
Shi, Jin;McLamore, Eric S.;Porterfield, D. Marshall
生理研究需要灵敏的工具来直接量化生理条件下细胞/组织空间域的转运动力学。虽然生物传感器能够测量浓度，但由于相对较低的灵敏度、过多的漂移/噪声以及无法量化分析物的运输，它们在生理研究中的应用受到限制。纳米材料显著改善了微电极的电化学转导，使高灵敏度微生物传感器的构建成为可能。此外，一种新的生物传感器模式，自参考（SR），可以直接测量实时通量和漂移/噪声减法。基于纳米材料的SR微生物传感器已被用于测量几种细胞/组织研究中与各种刺激剂/抑制剂耦合的实时分析物运输。这些研究包括：胰腺β细胞、癌细胞、肌肉组织、肠组织和铜绿假单胞菌生物膜的葡萄糖摄取；神经细胞附近谷氨酸通量；玉米根系表面内源吲哚-3-乙酸通量。SR研究结果为动态生理条件下的癌症、糖尿病、营养、神经生理、环境和植物生理研究提供了重要的见解，表明SR微生物传感器在生理学研究中具有极其重要的价值。
Physiological studies require sensitive tools to directly quantify transport kinetics in the cell/tissue spatial domain under physiological conditions. Although biosensors are capable of measuring concentration, their applications in physiological studies are limited due to the relatively low sensitivity, excessive drift/noise, and inability to quantify analyte transport. Nanomaterials significantly improve the electrochemical transduction of microelectrodes, and make the construction of highly sensitive microbiosensors possible. Furthermore, a novel biosensor modality, self-referencing (SR), enables direct measurement of real-time flux and drift/noise subtraction. SR microbiosensors based on nanomaterials have been used to measure the real-time analyte transport in several cell/tissue studies coupled with various stimulators/inhibitors. These studies include: glucose uptake in pancreatic β cells, cancer cells, muscle tissues, intestinal tissues and P. Aeruginosa biofilms; glutamate flux near neuronal cells; and endogenous indole-3-acetic acid flux near the surface of Zea mays roots. Results from the SR studies provide important insights into cancer, diabetes, nutrition, neurophysiology, environmental and plant physiology studies under dynamic physiological conditions, demonstrating that the SR microbiosensors are an extremely valuable tool for physiology research.
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影响因子：
3.3
作者：
Chakrapani, N;Zhang, YMM;Ajayan, PM
通讯作者：
Ajayan, PM
DOI：
10.1073/pnas.71.9.3405
发表时间：
1974-01-01
影响因子：
11.1
作者：
HELLMAN, B;IDAHL, LA;TALJEDAL, IB
通讯作者：
TALJEDAL, IB
影响因子：
7.7
作者：
Kennedy, RT;Kauri, LM;Jung, SK
通讯作者：
Jung, SK
DOI：
10.1083/jcb.110.5.1565
发表时间：
1990-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kühtreiber WM;Jaffe LF
通讯作者：
Jaffe LF
DOI：
10.1006/bbrc.1996.1622
发表时间：
1996-11-01
影响因子：
3.1
作者：
Barbosa, RM;Silva, AM;Rosario, LM
通讯作者：
Rosario, LM