Monosynaptic convergence of chorda tympani and glossopharyngeal afferents onto ascending relay neurons in the nucleus of the solitary tract: a high-resolution confocal and correlative electron microscopy approach.
Monosynaptic convergence of chorda tympani and glossopharyngeal afferents onto ascending relay neurons in the nucleus of the solitary tract: a high-resolution confocal and correlative electron microscopy approach.
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DOI：
10.1002/cne.23357
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发表时间：
2013-09-01
影响因子：
2.5
通讯作者：
Erisir, Alev
中科院分区：
文献类型：
--
作者：
Corson, James A.;Erisir, Alev
虽然生理学研究表明鼓索和舌咽传入轴突汇聚到孤束喙核（rNTS）的单个神经元上，但解剖学证据一直难以捉摸。目前的研究使用高放大倍率共聚焦显微镜来识别从两个神经的传入纤维到单个投射神经元的假定突触接触。使用电子显微镜重新可视化成像组织，确认共焦 z 堆栈中重叠的荧光信号准确识别标记末端和树突对之间的对位。蒙特卡洛模型揭示了荧光团重叠的概率与传入标签的密度随机无关，这表明 rNTS 中的收敛神经支配是选择性的而不是机会性的。每个神经的假定突触接触通常被划分到集中神经支配的神经元的树突段上。这些结果对 rNTS 中的口腔感觉处理具有重要意义，这里介绍的技术可应用于神经微电路的研究，重点是神经支配模式。
While physiological studies suggested convergence of chorda tympani and glossopharyngeal afferent axons onto single neurons of the rostral nucleus of the solitary tract (rNTS), anatomical evidence has been elusive. The current study uses high-magnification confocal microscopy to identify putative synaptic contacts from afferent fibers of the two nerves onto individual projection neurons. Imaged tissue is re-visualized with electron microscopy, confirming that overlapping fluorescent signals in confocal z-stacks accurately identify appositions between labeled terminal and dendrite pairs. Monte Carlo modeling reveals that the probability of overlapping fluorophores is stochastically unrelated to the density of afferent label suggesting that convergent innervation in the rNTS is selective rather than opportunistic. Putative synaptic contacts from each nerve are often compartmentalized onto dendrite segments of convergently innervated neurons. These results have important implications for orosensory processing in the rNTS, and the techniques presented here have applications in investigations of neural microcircuitry with an emphasis on innervation patterning.
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影响因子：
3
作者：
Cabirol-Pol, MJ;Mizrahi, A;Meyrand, P
通讯作者：
Meyrand, P
影响因子：
16.2
作者：
Li, Jianli;Erisir, Alev;Cline, Hollis
通讯作者：
Cline, Hollis
影响因子：
2.9
作者：
MATSUO, R;INOUE, T;MORIMOTO, T
通讯作者：
MORIMOTO, T
DOI：
10.1523/jneurosci.5995-08.2009
发表时间：
2009-07-22
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Di Lorenzo PM;Chen JY;Victor JD
通讯作者：
Victor JD
影响因子：
2.5
作者：
Breza, JM;Curtis, KS;Contreras, RJ
通讯作者：
Contreras, RJ