Endoplasmic reticulum-resident Rab8A GTPase is involved in phagocytosis in the protozoan parasite Entamoeba histolytica.
Endoplasmic reticulum-resident Rab8A GTPase is involved in phagocytosis in the protozoan parasite Entamoeba histolytica.
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DOI：
10.1111/cmi.12570
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发表时间：
2016-10
影响因子：
3.4
通讯作者：
Nozaki T
中科院分区：
文献类型：
--
作者：
Hanadate Y;Saito-Nakano Y;Nakada-Tsukui K;Nozaki T
吞噬作用在肠道原虫溶组织内阿米巴寄生虫的发病机制中是不可或缺的。在本研究中，我们发现在溶组织单胞杆菌中，Rab8A主要存在于内质网（ER）中，并参与吞噬作用。Rab8A在其他生物体中通常参与从反高尔基网络到质膜的运输，但之前在蛋白质组学分析中在阿米巴原虫的吞噬体中被发现。我们证明了通过小反义RNA介导的转录基因沉默来下调EhRab8A显著降低红细胞、细菌和羧化乳胶珠的粘附和吞噬。表面生物素化后的SDS - PAGE分析显示，在EhRab8A基因沉默菌株中，几种可能参与目标识别的蛋白质的表面表达减少。此外，野生型EhRab8A的过表达增强了吞噬作用，而显性阴性EhRab8A的表达则导致吞噬作用减少。这些结果表明EhRab8A调节猎物表面受体从内质网到质膜的转运。据我们所知，这是首次报道内质网驻地Rab GTPase通过调节表面受体的运输参与吞噬，支持内质网直接参与吞噬的前提。
Phagocytosis is indispensable for the pathogenesis of the intestinal protozoan parasite Entamoeba histolytica. Here, we showed that in E. histolytica Rab8A, which is generally involved in trafficking from the trans‐Golgi network to the plasma membrane in other organisms but was previously identified in phagosomes of the amoeba in the proteomic analysis, primarily resides in the endoplasmic reticulum (ER) and participates in phagocytosis. We demonstrated that down‐regulation of EhRab8A by small antisense RNA‐mediated transcriptional gene silencing remarkably reduced adherence and phagocytosis of erythrocytes, bacteria and carboxylated latex beads. Surface biotinylation followed by SDS‐PAGE analysis revealed that the surface expression of several proteins presumably involved in target recognition was reduced in the EhRab8A gene‐silenced strain. Further, overexpression of wild‐type EhRab8A augmented phagocytosis, whereas expression of the dominant‐negative form of EhRab8A resulted in reduced phagocytosis. These results indicated that EhRab8A regulates transport of surface receptor(s) for the prey from the ER to the plasma membrane. To our knowledge, this is the first report that the ER‐resident Rab GTPase is involved in phagocytosis through the regulation of trafficking of a surface receptor, supporting a premise of direct involvement of the ER in phagocytosis.
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影响因子：
6.7
作者：
Bracha R;Nuchamowitz Y;Anbar M;Mirelman D
通讯作者：
Mirelman D
影响因子：
5.4
作者：
DIAMOND, LS;BARTGIS, IL;MATTERN, CFT
通讯作者：
MATTERN, CFT
影响因子：
--
作者：
Bracha, Rivka;Nuchamowitz, Yael;Mirelman, David
通讯作者：
Mirelman, David
DOI：
10.1074/mcp.m113.031393
发表时间：
2014-01
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Biller L;Matthiesen J;Kühne V;Lotter H;Handal G;Nozaki T;Saito-Nakano Y;Schümann M;Roeder T;Tannich E;Krause E;Bruchhaus I
通讯作者：
Bruchhaus I
影响因子：
64.5
作者：
DER, CJ;FINKEL, T;COOPER, GM
通讯作者：
COOPER, GM