Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation
Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation
复制标题
去神经后人喉外展肌肌球蛋白重链亚型的转变
DOI：
10.1007/s00405-015-3664-z
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发表时间：
2015-06
影响因子：
2.6
通讯作者：
Chen Shicai
中科院分区：
文献类型：
--
作者：
Qiu Xiaoxia;Chen Donghui;Li Meng;Gao Yingna;Liu Fei;Zheng Hongliang;Chen Shicai
本研究的目的是探讨喉返神经（RLN）损伤后人环杓后肌（PCA）肌纤维亚型的转变。从38例双侧声带麻痹患者获取了PCA肌肉标本。
The objective of this study was to investigate the myofiber subtype transition of human posterior cricoarytenoid (PCA) muscle after the injury to recurrent laryngeal nerve (RLN). PCA muscle specimens were obtained from 38 bilateral vocal fold paralysis patients underwent arytenoidectomy. According to the duration of RLN injury, all the cases were divided into five denervation groups: 6–12 months, 1–2, 2–3, 3–6, and >6 years. The normal PCA muscles from total laryngectomy patients were chosen as controls. Immunofluorescence was adopted to detect the expression level of myosin heavy chain (MHC)-I and MHC-II in PCA muscle. Quantitative real-time PCR was also used to assess the transcriptional level of MHC subtypes (MHC-I, MHC-IIa, MHC-IIb, MHC-IIx, embryonic-MHC, and peri-natal-MHC). Immunofluorescence showed that MHC-I-positive myofibers in denervation groups were much lower than control group, respectively, while MHC-II-positive myofibers were significantly higher than control group (P< 0.05). With the extension of denervation, the number of MHC-I-positive myofibers gradually decreased, while MHC-II gradually increased and peaked in 1- to 2-year group. Transcriptional level of MHC-I, MHC-IIa, and MHC-IIb in denervation groups significantly down-regulated compared with the control (P< 0.05), respectively. However, MHC-IIx, embryonic-MHC, and peri-natal-MHC significantly up-regulated in all denervation groups, and the highest level was in 1- to 2-year denervation group. Data from the present study demonstrated that the maximum transition of MHC subtypes in human PCA muscles occurred in 1–2 years after denervation, suggesting that laryngeal reinnervation before the occurrence of irreversible transition of MHC subtypes could maintain the structural integrity of laryngeal PCA muscles.
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影响因子：
5.5
作者：
BULLER, AJ;ECCLES, JC;ECCLES, RM
通讯作者：
ECCLES, RM
DOI：
10.1097/00005537-199909000-00030
发表时间：
1999-09
期刊：
The Laryngoscope
影响因子：
--
作者：
A. Shiotani;W. Westra;P. Flint
通讯作者：
A. Shiotani;W. Westra;P. Flint
影响因子：
2.4
作者：
Adreani, C. M.;Li, Z. B.;Parente, E. J.
通讯作者：
Parente, E. J.
影响因子：
2.6
作者：
Shiotani, A;Flint, PW
通讯作者：
Flint, PW
DOI：
10.1001/archotol.130.9.1070
发表时间：
2004-09
期刊：
Archives of otolaryngology--head & neck surgery
影响因子：
--
作者：
Y. Z. Wu;M. Baker;J. Marie;R. Crumley;V. Caiozzo
通讯作者：
Y. Z. Wu;M. Baker;J. Marie;R. Crumley;V. Caiozzo