Molecular Mapping of QTLs Associated with Lodging Resistance in Dry Direct-Seeded Rice (Oryza sativa L.).
Molecular Mapping of QTLs Associated with Lodging Resistance in Dry Direct-Seeded Rice (Oryza sativa L.).
复制标题
QTL的分子映射与干米（Oryza sativa L.）中的耐药性相关。
DOI：
10.3389/fpls.2017.01431
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发表时间：
2017
影响因子：
5.6
通讯作者：
Kumar A
中科院分区：
文献类型：
--
作者：
Yadav S;Singh UM;Naik SM;Venkateshwarlu C;Ramayya PJ;Raman KA;Sandhu N;Kumar A
水稻旱直播是一种通过机械化进行的节水、省工的作物栽培方式。它为种植季节的第二季作物提供了更好的机会，因此，它是一种可行的替代低地移栽水稻的系统。然而，倒伏是水稻高产的主要制约因素之一。鉴定抗倒伏QTL并利用其在DSR下改良品种将是解决这一问题的有效育种策略。为了定位与抗倒性相关的QTL，连续两年对253个Swarna和Moroberekan回交后代BC 3F 4株系进行了分析。利用193个多态性SNP标记，将12个与抗倒伏性状相关的QTL定位在第1、2、6和7染色体上。在第1染色体上发现了2个主效QTL，即qCD1.1（R2为10%）和qCS1.1（R2为14%），其中id 1003559为峰值SNP标记（侧翼标记; id 1001973-id 1006772），它们是与重要抗倒伏性状相关的共同基因组区域。计算机模拟分析显示，在id 1001973-id 1006772的标记间隔区域中存在赤霉素3 β-羟化酶沿着以及34个其他推定的候选基因。秆长、秆径和秆力的正等位基因由高地适应性亲本Moroberekan贡献。研究结果表明，水稻抗倒性状（秆长、直径和比强度）与水稻产量呈显著正相关，表明水稻抗倒性状在水稻增产中的作用。在分子标记辅助导入程序中利用已鉴定的影响秆强和秆粗的等位基因可能有助于DSR条件下的抗倒伏性。
Dry direct-seeded rice (DSR) is an alternative crop establishment method with less water and labor requirement through mechanization. It provides better opportunities for a second crop during the cropping season and therefore, a feasible alternative system to transplanted lowland rice. However, lodging is one of the major constraints in attaining high yield in DSR. Identification of QTLs for lodging resistance and their subsequent use in improving varieties under DSR will be an efficient breeding strategy to address the problem. In order to map the QTLs associated with lodging resistance, a set of 253 BC3F4 lines derived from a backcross between Swarna and Moroberekan were evaluated in two consecutive years. A total of 12 QTLs associated with lodging resistance traits [culm length (qCL), culm diameter (qCD), and culm strength (qCS)] were mapped on chromosomes 1, 2, 6, and 7 using 193 polymorphic SNP markers. Two major and consistent effect QTLs, namely qCD1.1 (with R2 of 10%) and qCS1.1 (with R2 of 14%) on chromosome 1 with id1003559 being the peak SNP marker (flanking markers; id1001973-id1006772) were identified as a common genomic region associated with important lodging resistance traits. In silico analysis revealed the presence of Gibberellic Acid 3 beta-hydroxylase along with 34 other putative candidate genes in the marker interval region of id1001973-id1006772. The positive alleles for culm length, culm diameter, and culm strength were contributed by the upland adaptive parent Moroberekan. Our results identified significant positive correlation between lodging related traits (culm length diameter and strength) and grain yield under DSR, indicating the role of lodging resistant traits in grain yield improvement under DSR. Deployment of the identified alleles influencing the culm strength and culm diameter in marker assisted introgression program may facilitate the lodging resistance under DSR.
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影响因子：
3.7
作者：
Matsubara K;Yamamoto E;Kobayashi N;Ishii T;Tanaka J;Tsunematsu H;Yoshinaga S;Matsumura O;Yonemaru J;Mizobuchi R;Yamamoto T;Kato H;Yano M
通讯作者：
Yano M
影响因子：
2
作者：
Berry, PM;Spink, JH;Craigon, J
通讯作者：
Craigon, J
影响因子：
2.3
作者：
Flint-Garcia, SA;Jampatong, C;McMullen, MD
通讯作者：
McMullen, MD
影响因子：
1.9
作者：
Hai, L;Guo, HH;Jia, JZ
通讯作者：
Jia, JZ
影响因子：
7.4
作者：
Kashiwagi, T;Ishimaru, K
通讯作者：
Ishimaru, K