Folic acid reduces the ethanol-induced morphological and behavioral defects in embryonic and larval zebrafish (Danio rerio) as a model for fetal alcohol spectrum disorder (FASD).
Folic acid reduces the ethanol-induced morphological and behavioral defects in embryonic and larval zebrafish (Danio rerio) as a model for fetal alcohol spectrum disorder (FASD).
复制标题
叶酸减少了乙醇诱导的胚胎和幼虫斑马鱼（Danio Rerio）的形态和行为缺陷，作为胎儿酒精谱系障碍（FASD）的模型。
DOI：
10.1016/j.reprotox.2020.07.013
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发表时间：
2020-09
期刊：
影响因子：
--
通讯作者：
Marrs JA
中科院分区：
文献类型：
--
作者：
Cadena PG;Cadena MRS;Sarmah S;Marrs JA
这项工作的目的是确定是否叶酸（FA）减少胚胎乙醇（EtOH）暴露诱导的行为和形态缺陷，在我们的斑马鱼胎儿酒精谱系障碍（FASD）模型。使用四种处理条件：未处理、FA、EtOH和EtOH+FA，在幼虫（受精后6-7天）中评价致畸作用、死亡率、兴奋性明暗运动（ELD）、睡眠（SL）、趋触性（TH）、触觉敏感度（TS）和视动反应（OMR）试验。FA减少了在EtOH暴露的鱼中观察到的心脏、眼睛和鱼鳔膨胀的形态缺陷。幼虫在黑暗中比在光照条件下更活跃，EtOH降低了ELD测试中的游泳活动。乙醇影响了睡眠模式，诱导了几个觉醒期，增加了斑马鱼的不活动。FA减少了这些毒性作用，并在夜间产生更一致的不活动，减少了唤醒期。FA还防止EtOH诱导的幼虫趋触性和视动反应的缺陷。我们的结论是，在这个FASD模型中，乙醇暴露产生了几个致畸和行为缺陷，FA减少，但没有完全防止，这些缺陷。了解EtOH诱导的行为缺陷可能有助于确定FASD的新治疗或预防策略。
The objective of this work was to determine whether folic acid (FA) reduces the embryonic ethanol (EtOH) exposure induced behavioral and morphological defects in our zebrafish fetal alcohol spectrum disorder (FASD) model. Teratogenic effects, mortality, the excitatory light-dark locomotion (ELD), sleep (SL), thigmotaxis (TH), touch sensitivity (TS), and optomotor response (OMR) tests were evaluated in larvae (6–7 days post-fertilization) using four treatment conditions: Untreated, FA, EtOH and EtOH+FA. FA reduced morphological defects on heart, eyes and swim bladder inflation seen in EtOH exposed fish. The larvae were more active in the dark than in light conditions, and EtOH reduced the swimming activity in the ELD test. EtOH affected the sleep pattern, inducing several arousal periods and increasing inactivity in zebrafish. FA reduces these toxic effects and produced more consistent inactivity during the night, reducing the arousal periods. FA also prevented the EtOH-induced defects in thigmotaxis and optomotor response of the larvae. We conclude that in this FASD model, EtOH exposure produced several teratogenic and behavioral defects, FA reduced, but did not totally prevent, these defects. Understanding of EtOH-induced behavioral defects could help to identify new therapeutic or prevention strategies for FASD.
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影响因子：
3.3
作者：
Muralidharan P;Sarmah S;Zhou FC;Marrs JA
通讯作者：
Marrs JA
影响因子：
2.5
作者：
Sarmah, Swapnalee;Marrs, James A.
通讯作者：
Marrs, James A.
影响因子：
3.4
作者：
MacPhail, R. C.;Brooks, J.;Padilla, S.
通讯作者：
Padilla, S.
DOI：
10.1016/j.pnpbp.2019.109728
发表时间：
2020-01-10
影响因子：
5.6
作者：
Collier, Adam D.;Min, Soe S.;Leibowitz, Sarah F.
通讯作者：
Leibowitz, Sarah F.
影响因子：
2.4
作者：
Sarmah, Swapnalee;Muralidharan, Pooja;Marrs, James A.
通讯作者：
Marrs, James A.