Comparative Transcriptome Analysis Reveals the Interaction of Sugar and Hormone Metabolism Involved in the Root Hair Morphogenesis of the Endangered Fir Abies beshanzuensis.
Comparative Transcriptome Analysis Reveals the Interaction of Sugar and Hormone Metabolism Involved in the Root Hair Morphogenesis of the Endangered Fir Abies beshanzuensis.
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比较转录组分析揭示了濒危冷杉根毛形态发生中糖和激素代谢的相互作用
DOI：
10.3390/plants12020276
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发表时间：
2023-01-06
期刊：
影响因子：
--
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Liu B;Liu K;Chen X;Xiao D;Wang T;Yang Y;Shuai H;Wu S;Yuan L;Chen L
百山祖冷杉是世界上仅有3株野生成木的珍稀濒危植物，具有强烈的菌根依赖性，在没有共生关系的情况下，很难进行保护和人工繁育。根毛形态发生对菌根共生体的生存起着重要作用。由于缺乏有效的基因组和转录组的A。百山祖毛根发育的分子信号尚不清楚，阻碍了其濒危机制的分析和保护。本文对A.初步建立了百山祖试管苗。功能注释和差异表达基因（DEG）分析表明，两种表型具有高度差异表达的基因簇。转录组分化确定激素和糖信号主要参与根毛的形态发生。beshanzuensis。加权相关网络分析（WGCNA）结合实时荧光定量PCR（qRT-PCR）发现，蔗糖-根毛两个模块由IAA17连接，SUS位于调控网络的中心，与激素转导中的SRK2E和根毛形态发生相关的关键基因共表达。我们的研究结果有助于更好地理解根毛发育的分子机制，并为破译A. beshanzuensis和其他濒危物种，利用根毛作为补偿策略，而不是穷人菌根生长。
Abies beshanzuensis, an extremely rare and critically endangered plant with only three wild adult trees globally, is strongly mycorrhizal-dependent, leading to difficulties in protection and artificial breeding without symbiosis. Root hair morphogenesis plays an important role in the survival of mycorrhizal symbionts. Due to the lack of an effective genome and transcriptome of A. beshanzuensis, the molecular signals involved in the root hair development remain unknown, which hinders its endangered mechanism analysis and protection. Herein, transcriptomes of radicles with root hair (RH1) and without root hair (RH0) from A. beshanzuensis in vitro plantlets were primarily established. Functional annotation and differentially expressed gene (DEG) analysis showed that the two phenotypes have highly differentially expressed gene clusters. Transcriptome divergence identified hormone and sugar signaling primarily involved in root hair morphogenesis of A. beshanzuensis. Weighted correlation network analysis (WGCNA) coupled with quantitative real-time PCR (qRT-PCR) found that two hormone–sucrose–root hair modules were linked by IAA17, and SUS was positioned in the center of the regulation network, co-expressed with SRK2E in hormone transduction and key genes related to root hair morphogenesis. Our results contribute to better understanding of the molecular mechanisms of root hair development and offer new insights into deciphering the survival mechanism of A. beshanzuensis and other endangered species, utilizing root hair as a compensatory strategy instead of poor mycorrhizal growth.
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影响因子：
4.5
作者：
Bruex A;Kainkaryam RM;Wieckowski Y;Kang YH;Bernhardt C;Xia Y;Zheng X;Wang JY;Lee MM;Benfey P;Woolf PJ;Schiefelbein J
通讯作者：
Schiefelbein J
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
2.8
作者：
Harigaya, Wakana;Takahashi, Hidenori
通讯作者：
Takahashi, Hidenori
DOI：
10.1073/pnas.1711723115
发表时间：
2017-12-26
影响因子：
11.1
作者：
Feng, Ying;Xu, Ping;Guo, Hongwei
通讯作者：
Guo, Hongwei
DOI：
10.3390/plants10010111
发表时间：
2021-01-07
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
García-González J;Lacek J;Retzer K
通讯作者：
Retzer K