Copper selects for siderophore-mediated virulence in Pseudomonas aeruginosa.
Copper selects for siderophore-mediated virulence in Pseudomonas aeruginosa.
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DOI：
10.1186/s12866-022-02720-w
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发表时间：
2022-12-13
期刊：
影响因子：
4.2
通讯作者：
Vos, Michiel
中科院分区：
文献类型：
--
作者：
Lear, Luke;Hesse, Elze;Buckling, Angus;Vos, Michiel
关键词：
铁对几乎所有细菌病原体都是必不可少的，因此它被它们的宿主积极地保留。然而，细胞外铁载体的产生使得病原体能够螯合铁，增加它们的毒力。铁载体的另一个功能是非铁金属的细胞外解毒。在这里，我们通过测试条件致病菌铜绿假单胞菌在接触铜后是否表现出更大的毒力，实验性地将铁载体的解毒作用和毒力作用联系起来。为此，我们将铜绿假单胞菌在不同的环境相关铜方案下孵育两天或十二天。随后在无铜环境中的生长去除了表型效应，然后我们使用大蜡螟感染模型定量绿脓菌荧光素产生（由铜绿假单胞菌产生的主要铁载体）和毒力。铜选择增加绿脓菌荧光素生产，这是正相关的毒力。这种影响随着时间的推移而增加，这样，与高铜孵育12天的种群是最致命的。用铜绿假单胞菌的不产生绿脓菌荧光素的菌株进行的实验的重复证明绿脓菌荧光素的产生在很大程度上是毒力变化的原因。我们在这里展示了金属压力和细菌毒力之间的直接联系，突出了金属污染对人类健康有害影响的另一个方面。
Iron is essential for almost all bacterial pathogens and consequently it is actively withheld by their hosts. However, the production of extracellular siderophores enables iron sequestration by pathogens, increasing their virulence. Another function of siderophores is extracellular detoxification of non-ferrous metals. Here, we experimentally link the detoxification and virulence roles of siderophores by testing whether the opportunistic pathogen Pseudomonas aeruginosa displays greater virulence after exposure to copper. To do this, we incubated P. aeruginosa under different environmentally relevant copper regimes for either two or twelve days. Subsequent growth in a copper-free environment removed phenotypic effects, before we quantified pyoverdine production (the primary siderophore produced by P. aeruginosa), and virulence using the Galleria mellonella infection model. Copper selected for increased pyoverdine production, which was positively correlated with virulence. This effect increased with time, such that populations incubated with high copper for twelve days were the most virulent. Replication of the experiment with a non-pyoverdine producing strain of P. aeruginosa demonstrated that pyoverdine production was largely responsible for the change in virulence. We here show a direct link between metal stress and bacterial virulence, highlighting another dimension of the detrimental effects of metal pollution on human health.
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影响因子：
8.9
作者：
Brun, LA;Maillet, J;Remy, JC
通讯作者：
Remy, JC
DOI：
10.1038/ismej.2017.103
发表时间：
2017-11
期刊：
The ISME journal
影响因子：
--
作者：
Harrison F;McNally A;da Silva AC;Heeb S;Diggle SP
通讯作者：
Diggle SP
影响因子：
4.9
作者：
Figueiredo, Alexandre R. T.;Wagner, Andreas;Kummerli, Rolf
通讯作者：
Kummerli, Rolf
影响因子：
11
作者：
Harrison, Freya;Paul, Jon;Buckling, Angus
通讯作者：
Buckling, Angus
DOI：
10.1073/pnas.1914830117
发表时间：
2020-03-31
影响因子：
11.1
作者：
Iatsenko, Igor;Marra, Alice;Lemaitre, Bruno
通讯作者：
Lemaitre, Bruno