Identification of Changing Ribosome Protein Compositions using Mass Spectrometry.
Identification of Changing Ribosome Protein Compositions using Mass Spectrometry.
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DOI：
10.1002/pmic.201800217
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发表时间：
2018-10
期刊：
影响因子：
3.4
通讯作者：
Link AJ
中科院分区：
文献类型：
--
作者：
Samir P;Browne CM;Rahul;Sun M;Shen B;Li W;Frank J;Link AJ
核糖体在基因表达中的调节作用已成为人们关注的焦点。核糖体是一种动态复合体，能够改变其蛋白质组成以响应环境刺激。我们应用质谱仪来鉴定酿酒酵母80S核糖体在不同环境刺激下蛋白质组成的定量变化。利用定量质谱仪，我们发现当酵母从葡萄糖中生长转变为甘油生长时，平行酵母核糖体蛋白RPL8A(EL8A)和RPL8B(EL8B)在80s核糖体中的相对比例发生了变化。利用酵母遗传学和多聚体图谱，我们证明了含有RPL8A或RPL8B的酵母核糖体在功能上是不可互换的。我们的定量蛋白质组数据支持这样的假设，即核糖体是动态的复合体，可以随着生长或环境条件的变化而改变其组成和功能活性。
The regulatory role of the ribosome in gene expression has come into sharper focus. It has been proposed that ribosomes are dynamic complexes capable of changing their protein composition in response to enviromental stimuli. We applied mass spectrometry to identify quantitative changes in the protein composition of S. cerevisiae 80S ribosomes in response to different environmental stimuli. Using quantitative mass spectrometry, we found that the paralog yeast ribosomal proteins RPL8A (eL8A) and RPL8B (eL8B) change their relative proportions in the 80S ribosome when yeast is switched from growth in glucose to glycerol. Using yeast genetics and polysome profiling, we show that yeast ribosomes containing either RPL8A or RPL8B are not functionally interchangeable. Our quantitative proteomic data support the hypothesis that ribosomes are dynamic complexes that alter their composition and functional activity in response to changes in growth or environmental conditions.
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影响因子：
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