Zebrafish Transcription Factor ORFeome for Gene Discovery and Regulatory Network Elucidation.

Zebrafish Transcription Factor ORFeome for Gene Discovery and Regulatory Network Elucidation.
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用于基因发现和调控网络阐明的斑马鱼转录因子 ORFeome。

DOI:
10.1089/zeb.2017.1486
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发表时间:
2018
期刊:
影响因子:
2
通讯作者:
Breton,Ghislain
Breton,Ghislain
中科院分区:
生物学4区
文献类型:
--
作者:
King,Justin;Foster,Justin;Davison,JamesM;Rawls,JohnF;Breton,Ghislain

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斑马鱼基因组序列的完成以及小型化和多路复用的进展对于创建RNA-seq,ChIP-seq以及高通量行为和化学筛选等技术至关重要。多重化也有助于最近增强经典的酵母单杂交相互作用技术,为蛋白质-DNA相互作用提供前所未有的发现能力。不幸的是,它用于斑马鱼研究目前受到缺乏一个开放的阅读框架(ORF)克隆收集。作为完整收集的第一步,我们描述了一个包含142个ORF的转录调控蛋白的小型文库及其潜在的应用。
The completion of the zebrafish genome sequence and advances in miniaturization and multiplexing were essential to the creation of techniques such as RNA-seq, ChIP-seq, and high-throughput behavioral and chemical screens. Multiplexing was also instrumental in the recent enhancement of the classic yeast one-hybrid interaction techniques to provide unprecedented discovery capabilities for protein–DNA interactions. Unfortunately its use for zebrafish research is currently hampered by the lack of an open reading frame (ORF) clone collection. As a first step toward a complete collection, we describe a small library of transcriptional regulatory proteins comprising 142 ORFs and its potential applications.
DOI: 10.1093/nar/gkx682
发表时间: 2017-10-13
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