Liquid-solid phase-inversion PLGA implant for the treatment of residual tumor tissue after HIFU ablation.
Liquid-solid phase-inversion PLGA implant for the treatment of residual tumor tissue after HIFU ablation.
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液固相反转 PLGA 植入物治疗 HIFU 消融后残留肿瘤组织
DOI：
10.1371/journal.pone.0117358
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Li J;Krupka T;Yao J;Wang R;Jiang L;Zhou Y;Zuo G;Wang Z;Dai L;Ren J;Zheng Y;Wang D
HIFU由于其非侵入性和体外性，已被证明是治疗原发性实体瘤和转移性疾病比其他局灶性热消融技术更合适的选择。然而，与其他局灶性热消融技术类似，由于肿瘤复发，HIFU仍需要改进。在这项工作中，我们研究了一种辅助治疗方案的有效性，该方案使用负载多柔比星（DOX）、可注射、原位形成和相位逆转的PLGA作为HIFU消融后的第二道防线，以破坏有害残留肿瘤并防止肿瘤复发。所有统计分析均使用社会科学统计软件包18.0（SPSS, Inc., Chicago， IL， USA）进行，p< 0.05被认为具有统计学意义。所有结果均以均数±STDEV（标准差）表示。对于多重比较，当数据为正态分布且方差齐时，采用方差分析（ANOVA）（肿瘤体积、生长速率、细胞凋亡、增殖指数、Bcl-2和Bax蛋白水平的差异），否则采用秩和检验。一旦发现显著差异，则使用Student-t检验进行两组之间的比较。我们的研究结果显示，DOX扩散到消融组织区域之外，进入不受HIFU消融影响的肿瘤细胞。我们的研究结果还表明，HIFU与dox负载PLGA协同作用，导致HIFU消融组织以外区域的肿瘤细胞凋亡率显著提高，肿瘤细胞增殖率显著降低，从而导致肿瘤体积显著缩小（治疗组、假手术组和无治疗组的肿瘤体积分别为0.26±0.1、1.09±0.76和1.42±0.9cm3）。从这些结果中，我们得出结论，HIFU消融后病灶内注射dox负载PLGA明显比单独HIFU治疗实体瘤更有效。
HIFU has been shown to be a more suitable alternative for the treatment of primary solid tumors and metastatic diseases than other focal heat ablation techniques due to its noninvasive and extracorporeal nature. However, similar to other focal heat ablation techniques, HIFU is still in need of refinements due to tumor recurrence. In this work, we investigated the effectiveness of an adjunct treatment regimen using doxorubicin (DOX)-loaded, injectable, in situ-forming, and phase-inverting PLGA as the second line of defense after HIFU ablation to destroy detrimental residual tumors and to prevent tumor recurrence. All of the statistical analyses were performed using the Statistical Package for the Social Sciences 18.0(SPSS, Inc., Chicago, IL, USA), and p< 0.05 was considered statistically significant. All of the results are presented as the means ± STDEV (standard deviation). For multiple comparisons, ANOVA (differences in tumor volumes, growth rates, apoptosis, proliferation indexes, and Bcl-2 and Bax protein levels) was used when the data were normally distributed with homogenous variance, and rank sum tests were used otherwise. Once significant differences were detected, Student-t tests were used for comparisons between two groups. Our results revealed that DOX diffused beyond the ablated tissue regions and entered tumor cells that were not affected by the HIFU ablation. Our results also show that HIFU in concert with DOX-loaded PLGA led to a significantly higher rate of tumor cell apoptosis and a lower rate of tumor cell proliferation in the areas beyond the HIFU-ablated tissues and consequently caused significant tumor volume shrinkage (tumor volumes:0.26±0.1,1.09±0.76, and 1.42±0.9cm3 for treatment, sham, and no treatment control, respectively). From these results, we concluded that the intralesional injection of DOX-loaded PLGA after HIFU ablation is significantly more effective than HIFU alone for the treatment of solid tumors.
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影响因子：
10.8
作者：
Solorio, Luis;Babin, Brett M.;Patel, Ravi B.;Mach, Justyna;Azar, Nami;Exner, Agata A.
通讯作者：
Exner, Agata A.
影响因子：
12.4
作者：
Solorio L;Olear AM;Hamilton JI;Patel RB;Beiswenger AC;Wallace JE;Zhou H;Exner AA
通讯作者：
Exner AA
影响因子：
10.8
作者：
DesNoyer, JR;McHugh, AJ
通讯作者：
McHugh, AJ
气体微泡 — 生成用于目标温度的全氟己烷纳米乳剂 — 敏感超声检查和协同 HIFU 消融肿瘤
影响因子：
29.4
作者：
Zhou, Yang;Wang, Zhigang;Zheng, Yuanyi
通讯作者：
Zheng, Yuanyi
影响因子：
4.9
作者：
Patel, Ravi B.;Carlson, Angela N.;Solorio, Luis;Exner, Agata A.
通讯作者：
Exner, Agata A.