Transcriptome Dynamics of Epidermal Reprogramming during Direct Shoot Regeneration in Torenia fournieri.
Transcriptome Dynamics of Epidermal Reprogramming during Direct Shoot Regeneration in Torenia fournieri.
复制标题
DOI：
10.1093/pcp/pcab101
复制
发表时间：
2021-11-10
影响因子：
4.9
通讯作者：
Sugiyama M
中科院分区：
文献类型：
--
作者：
Morinaka H;Mamiya A;Tamaki H;Iwamoto A;Suzuki T;Kawamura A;Ikeuchi M;Iwase A;Higashiyama T;Sugimoto K;Sugiyama M
芽再生包括体细胞的重新编程和茎顶端分生组织的从头组织。在拟南芥研究得最好的不定芽再生模型系统中，再生是由生长素响应的多能性愈伤组织根据侧根发育途径从中柱或类中柱组织中形成的。相反，在细胞分裂素的作用下，不需要细胞分裂素干预愈伤组织块的形成，而是直接从Torenia ournieri(Torenia)茎段外植体的完全分化的表皮细胞中诱导不定芽的再生，但其分子机制尚不清楚。在这里，我们通过细胞学观察和转录组分析来描述这种直接的不定芽再生。结果表明，随着培养的进行，基因表达谱迅速变化，获得了多个器官/组织的混合特征，可能与表皮重编程有关。通过对拟南芥三种不同的愈伤组织诱导培养物(生长素诱导愈伤组织、伤伤诱导愈伤组织和原生质体培养)与Torenia茎段培养物的转录本比较，确定在四种培养体系中上调的基因都是细胞重编程的共同因子。这些最初的变化不依赖于细胞分裂素，随后是依赖于细胞分裂素的核仁发育和细胞周期的转录激活。后来，SAM调控基因高度表达，导致SAM在表皮层增殖细胞的焦点组织。我们的发现揭示了三个不同的阶段，不同的转录和调控特征，从表皮直接再生不定芽，这为进一步研究这一独特的培养系统中的不定芽再生提供了基础。
Shoot regeneration involves reprogramming of somatic cells and de novo organization of shoot apical meristems (SAMs). In the best-studied model system of shoot regeneration using Arabidopsis, regeneration is mediated by the auxin-responsive pluripotent callus formation from pericycle or pericycle-like tissues according to the lateral root development pathway. In contrast, shoot regeneration can be induced directly from fully differentiated epidermal cells of stem explants of Torenia fournieri (Torenia), without intervening the callus mass formation in culture with cytokinin; yet, its molecular mechanisms remain unaddressed. Here, we characterized this direct shoot regeneration by cytological observation and transcriptome analyses. The results showed that the gene expression profile rapidly changes upon culture to acquire a mixed signature of multiple organs/tissues, possibly associated with epidermal reprogramming. Comparison of transcriptomes between three different callus-inducing cultures (callus induction by auxin, callus induction by wounding and protoplast culture) of Arabidopsis and the Torenia stem culture identified genes upregulated in all the four culture systems as candidates of common factors of cell reprogramming. These initial changes proceeded independently of cytokinin, followed by cytokinin-dependent, transcriptional activations of nucleolar development and cell cycle. Later, SAM regulatory genes became highly expressed, leading to SAM organization in the foci of proliferating cells in the epidermal layer. Our findings revealed three distinct phases with different transcriptomic and regulatory features during direct shoot regeneration from the epidermis in Torenia, which provides a basis for further investigation of shoot regeneration in this unique culture system.
登录
查看更多内容
影响因子：
2.7
作者：
CHRISTIANSON, ML;WARNICK, DA
通讯作者：
WARNICK, DA
DOI：
10.1139/b74-109
发表时间：
1974-01-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
CHLYAH, H
通讯作者：
CHLYAH, H
影响因子：
7.2
作者：
Atta, Ramzy;Laurens, Lieve;Chriqui, Dominique
通讯作者：
Chriqui, Dominique
生长素响应因子 3 调节细胞分裂素生物合成对芽分生组织诱导的生长素和细胞分裂素反应模式
影响因子：
7.4
作者：
Cheng, Zhi Juan;Wang, Liang;Zhang, Xian Sheng
通讯作者：
Zhang, Xian Sheng
H3K27me3 的重编程对于从培养的拟南芥组织中获得多能性至关重要
影响因子：
4.5
作者：
He C;Chen X;Huang H;Xu L
通讯作者：
Xu L