Release and protein binding of components from resin based composites in native saliva and other extraction media.
Release and protein binding of components from resin based composites in native saliva and other extraction media.
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天然唾液和其他提取介质中树脂基复合材料成分的释放和蛋白质结合
DOI：
10.1016/j.dental.2015.01.016
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Högg C
中科院分区：
文献类型：
--
作者：
Rothmund L;Shehata M;Van Landuyt KL;Schweikl H;Carell T;Geurtsen W;Hellwig E;Hickel R;Reichl FX;Högg C
未聚合的单体和添加剂可以从树脂基复合材料（RBC）中释放出来，并进入人体。在这项研究中，从复合材料的成分结合到唾液蛋白和血浆protein.MethodsThe复合材料的研究是Admira®流，金星®钻石流，Filtek™最高XTE流，Tetric EvoCeram®，Tetric EvoFlow®。根据RBC制造商的说明聚合样品（n= 4）。将样品浸入天然唾液、无蛋白唾液（人工唾液）、水和乙酸乙酯中，并在37 ℃下孵育24 h或72 h。通过气相色谱/质谱法分析洗脱液。为了确定与唾液蛋白的结合，将天然唾液中检测到的（共聚）单体和添加剂的浓度分别与无蛋白唾液、水和乙酸乙酯中检测到的（共聚）单体和添加剂的浓度进行比较。为了评估TEGDMA、EGDMA、DEGDMA、PMGDMA、BPA和DCHP与人血清白蛋白（HSA）和人α1-酸性糖蛋白（AGP）的亲和力，血浆蛋白结合试验（ABNOVA，Transil XL PPB Prediction Kit TMP-0212-2096）。实验组之间的差异（p< 0.05）使用单因素方差分析（ANOVA）进行检验，然后进行Tukey分析。天然唾液中释放的GMA和CyHEMA显著低于无蛋白质唾液或水中释放的浓度（Admira®流：72小时后TEGDMA的浓度：0.08 mmol/L（天然唾液），0.34 mmol/L（无蛋白质唾液），0.39 mmol/L（水））。与其他提取介质相比，在天然唾液中释放的HEMA、EGDMA、DDDMA和CQ的浓度甚至保持在检测限以下。BPA结合率为95.2%重要性人工唾液或水作为提取介质不能反映体内真实的生理情况。唾液和血浆蛋白可能结合（共）单体和添加剂，从而可能导致RBC中可吸收物的体内生物利用度低于先前的设想。
ObjectivesUnpolymerized (co)monomers and additives can be released from resin based composites (RBCs) and can enter the human organism. In this study, the binding of ingredients from composites to salivary proteins and plasma proteins was investigated.MethodsThe composites investigated were Admira®flow, Venus®Diamond flow, Filtek™ Supreme XTE flow, Tetric EvoCeram®, Tetric EvoFlow®. The samples (n= 4) were polymerized according to the instructions of the manufacturer of RBCs. The samples were immersed into native saliva, protein-free saliva (artificial saliva), water and ethyl acetate, and incubated at 37 °C for 24 h or 72 h. The eluates were analyzed by gas chromatography/mass spectrometry. To determine the binding to salivary proteins, the concentration of (co)monomers and additives detected in native saliva was compared to the concentration of (co)monomers and additives detected in protein-free saliva, water and ethyl acetate respectively.To assess the affinity of TEGDMA, EGDMA, DEGDMA, PMGDMA, BPA, and DCHP to human serum albumin (HSA) and human α1-acid glycoprotein (AGP), a plasma protein binding assay (ABNOVA, Transil XL PPB Prediction Kit TMP-0212-2096) was performed.The statistical significance (p< 0.05) of the difference between the experimental groups was tested using the one-way-analysis of variance (ANOVA), followed by Tukey‘s analysis.ResultsThe concentration of TEGDMA, GMA and CyHEMA released in native saliva was significantly lower than the concentration released in protein-free saliva or water (Admira®flow: concentration of TEGDMA after 72 h: 0.08 mmol/L (native saliva), 0.34 mmol/L (protein-free saliva), 0.39 mmol/L (water)). The concentrations of HEMA, EGDMA, DDDMA and CQ released in native saliva remained even below the detection limit, compared to the other extraction media.Protein binding of the tested methacrylates to HSA + AGP was 82–85%, the binding of DCHP was 96.6%, and the binding of BPA was 95.2%.SignificanceArtificial saliva or water as extraction medium does not reflect the real physiological situation in the body. Salivary and plasma proteins may bind (co)monomers and additives and may thereby contribute to a lower bioavailability of leachables from RBCs in vivo than previously thought.
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