Differentially evolved genes of Salmonella pathogenicity islands: insights into the mechanism of host specificity in Salmonella.
Differentially evolved genes of Salmonella pathogenicity islands: insights into the mechanism of host specificity in Salmonella.
复制标题
DOI：
10.1371/journal.pone.0003829
复制
发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Chakravortty, Dipshikha
中科院分区：
文献类型：
--
作者：
Eswarappa, Sandeepa M.;Janice, Jessin;Nagarajan, Arvindhan G.;Balasundaram, Sudhagar V.;Karnam, Guruswamy;Dixit, Narendra M.;Chakravortty, Dipshikha
肠道沙门氏菌（S. enterica）包括许多在禽类和哺乳动物宿主中引起疾病的血清型。这些血清型在宿主范围和宿主适应程度上差异很大。肠链球菌血清型的宿主特异性似乎是一个复杂的现象，受感染过程不同阶段的多种因素的影响，这使得仅通过实验方法确定宿主特异性的原因变得困难。在这项研究中，我们采用了基于分子进化和系统发育的方法来鉴定可能在赋予肠球菌不同血清型宿主特异性中发挥重要作用的基因。这些基因在不同的肠链球菌血清型中“不同地进化”。这一“差异进化”基因列表包括编码沙门氏菌致病性岛1和2的转座子蛋白（SipD、SseC和sed）的基因，编码三型分泌系统的基因，编码抑制宿主细胞有丝分裂原激活的蛋白激酶途径的效应蛋白的基因，以及编码效应蛋白（SseF和SifA）的基因，这些基因对将含沙门氏菌的液泡放置在近核位置很重要。对这些“差异进化基因”已知功能的分析表明，这些基因的产物直接与宿主细胞相互作用并操纵其功能，从而赋予宿主特异性，至少部分地，在本研究中考虑的不同肠球菌血清型。
The species Salmonella enterica (S. enterica) includes many serovars that cause disease in avian and mammalian hosts. These serovars differ greatly in their host range and their degree of host adaptation. The host specificity of S. enterica serovars appears to be a complex phenomenon governed by multiple factors acting at different stages of the infection process, which makes identification of the cause/s of host specificity solely by experimental methods difficult. In this study, we have employed a molecular evolution and phylogenetics based approach to identify genes that might play important roles in conferring host specificity to different serovars of S. enterica. These genes are ‘differentially evolved’ in different S. enterica serovars. This list of ‘differentially evolved’ genes includes genes that encode translocon proteins (SipD, SseC and SseD) of both Salmonella pathogenicity islands 1 and 2 encoded type three secretion systems, sptP, which encodes an effector protein that inhibits the mitogen-activated protein kinase pathway of the host cell, and genes which encode effector proteins (SseF and SifA) that are important in placing the Salmonella-containing vacuole in a juxtanuclear position. Analysis of known functions of these ‘differentially evolved genes’ indicates that the products of these genes directly interact with the host cell and manipulate its functions and thereby confer host specificity, at least in part, to different serovars of S. enterica that are considered in this study.
登录
查看更多内容
影响因子：
4.8
作者：
Boucrot, E;Beuzón, CR;Méresse, S
通讯作者：
Méresse, S
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
3.4
作者：
Gerlach, Roman G.;Jaeckel, Daniela;Hensel, Michael
通讯作者：
Hensel, Michael
影响因子：
56.9
作者：
Boucrot, E;Henry, T;Méresse, S
通讯作者：
Méresse, S
影响因子：
4.5
作者：
Abrahams, Garth L.;Mueller, Petra;Hensel, Michael
通讯作者：
Hensel, Michael