Glucagon-like peptide 1 recruits microvasculature and increases glucose use in muscle via a nitric oxide-dependent mechanism.
Glucagon-like peptide 1 recruits microvasculature and increases glucose use in muscle via a nitric oxide-dependent mechanism.
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作者：
Chai W;Dong Z;Wang N;Wang W;Tao L;Cao W;Liu Z
胰高血糖素样肽1（GLP-1）增加组织葡萄糖摄取并引起不依赖于胰岛素的血管舒张。我们研究了GLP-1对肌肉微血管和葡萄糖摄取的影响。在确认GLP-1有效刺激内皮细胞中的一氧化氮（NO）合酶（NOS）磷酸化后，过夜禁食的成年雄性大鼠接受连续GLP-1输注（30 pmol/kg/min）2 h+或-NOS抑制。测定肌肉微血管血容量（MBV）、微血管血流速度（MFV）和微血管血流量（MBF）。另外的大鼠接受GLP-1或盐水30分钟，并测定肌肉胰岛素清除/摄取。GLP-1在30 min内急性增加肌肉MBV（P < 0.04），而不改变MFV或股血流量。这种作用在整个120分钟输注期间持续存在，导致肌肉MBF增加两倍以上（P < 0.02）。这些变化与血浆NO水平、肌肉间质氧饱和度、后腿葡萄糖提取和肌肉胰岛素清除/摄取的增加相一致。NOS抑制阻断了GLP-1介导的肌肉MBV、葡萄糖处置、NO产生和肌肉胰岛素清除/摄取的增加。总之，GLP-1通过NO依赖性机制急性募集微血管并增加肌肉中的基础葡萄糖摄取。因此，GLP-1可能通过扩大微血管内皮表面积来改善肌肉胰岛素作用。
Glucagon-like peptide 1 (GLP-1) increases tissue glucose uptake and causes vasodilation independent of insulin. We examined the effect of GLP-1 on muscle microvasculature and glucose uptake. After confirming that GLP-1 potently stimulates nitric oxide (NO) synthase (NOS) phosphorylation in endothelial cells, overnight-fasted adult male rats received continuous GLP-1 infusion (30 pmol/kg/min) for 2 h plus or minus NOS inhibition. Muscle microvascular blood volume (MBV), microvascular blood flow velocity (MFV), and microvascular blood flow (MBF) were determined. Additional rats received GLP-1 or saline for 30 min and muscle insulin clearance/uptake was determined. GLP-1 infusion acutely increased muscle MBV (P < 0.04) within 30 min without altering MFV or femoral blood flow. This effect persisted throughout the 120-min infusion period, leading to a greater than twofold increase in muscle MBF (P < 0.02). These changes were paralleled with increases in plasma NO levels, muscle interstitial oxygen saturation, hind leg glucose extraction, and muscle insulin clearance/uptake. NOS inhibition blocked GLP-1–mediated increases in muscle MBV, glucose disposal, NO production, and muscle insulin clearance/uptake. In conclusion, GLP-1 acutely recruits microvasculature and increases basal glucose uptake in muscle via a NO-dependent mechanism. Thus, GLP-1 may afford potential to improve muscle insulin action by expanding microvascular endothelial surface area.
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影响因子：
16.2
作者：
Keske MA;Clerk LH;Price WJ;Jahn LA;Barrett EJ
通讯作者：
Barrett EJ
影响因子：
8.2
作者：
Hattori, Y.;Jojima, T.;Hayashi, T.
通讯作者：
Hayashi, T.
血管紧张素 II 1 型和 2 型受体调节基底骨骼肌微血管容量和葡萄糖使用
影响因子：
8.3
作者：
Chai, Weidong;Wang, Wenhui;Liu, Zhenqi
通讯作者：
Liu, Zhenqi
影响因子：
7.7
作者：
Chai W;Wang W;Dong Z;Cao W;Liu Z
通讯作者：
Liu Z
影响因子：
5.8
作者：
Liu, Jia;Jahn, Linda A.;Liu, Zhenqi
通讯作者：
Liu, Zhenqi