Structure of a type IV secretion system core complex encoded by multi-drug resistance F plasmids.
Structure of a type IV secretion system core complex encoded by multi-drug resistance F plasmids.
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DOI：
10.1038/s41467-022-28058-5
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发表时间：
2022-01-19
影响因子：
16.6
通讯作者：
Hu B
中科院分区：
文献类型：
--
作者：
Liu X;Khara P;Baker ML;Christie PJ;Hu B
Bacterial type IV secretion systems (T4SSs) are largely responsible for the proliferation of multi-drug resistance. We solved the structure of the outer-membrane core complex (OMCCF) of a T4SS encoded by a conjugative F plasmid at <3.0 Å resolution by cryoelectron microscopy. The OMCCF consists of a 13-fold symmetrical outer ring complex (ORC) built from 26 copies of TraK and TraV C-terminal domains, and a 17-fold symmetrical central cone (CC) composed of 17 copies of TraB β-barrels. Domains of TraV and TraB also bind the CC and ORC substructures, establishing that these proteins undergo an intraprotein symmetry alteration to accommodate the C13:C17 symmetry mismatch. We present evidence that other pED208-encoded factors stabilize the C13:C17 architecture and define the importance of TraK, TraV and TraB domains to T4SSF function. This work identifies OMCCF structural motifs of proposed importance for structural transitions associated with F plasmid dissemination and F pilus biogenesis. Bacteria conjugatively transfer DNA through type IV secretion systems (T4SSs). Here, the authors report the structure of a T4SS outer-membrane core complex (OMCC), revealing how a distinct C13:C17 symmetry mismatch exhibited by peripheral ring and central cone substructures is accommodated.
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影响因子：
--
作者：
Koraimann, Gunther
通讯作者：
Koraimann, Gunther
影响因子：
16.6
作者：
Dix SR;Owen HJ;Sun R;Ahmad A;Shastri S;Spiewak HL;Mosby DJ;Harris MJ;Batters SL;Brooker TA;Tzokov SB;Sedelnikova SE;Baker PJ;Bullough PA;Rice DW;Thomas MS
通讯作者：
Thomas MS
DOI：
10.1073/pnas.0806786105
发表时间：
2008-11-18
影响因子：
11.1
作者：
Clarke, Margaret;Maddera, Lucinda;Silverman, Philip M.
通讯作者：
Silverman, Philip M.
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
影响因子：
3.2
作者：
Jakubowski, SJ;Cascales, E;Christie, PJ
通讯作者：
Christie, PJ