Spectra from 2.5-15 microm of tissue phantom materials, optical clearing agents and ex vivo human skin: implications for depth profiling of human skin.
Spectra from 2.5-15 microm of tissue phantom materials, optical clearing agents and ex vivo human skin: implications for depth profiling of human skin.
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2.5-15 微米组织模型材料、光学透明剂和离体人体皮肤的光谱：对人体皮肤深度分析的影响。
DOI：
10.1088/0031-9155/48/2/402
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发表时间：
2003
影响因子：
3.5
通讯作者：
Nelson,JStuart
中科院分区：
文献类型：
--
作者：
Viator,JohnA;Choi,Bernard;Peavy,GeorgeM;Kimel,Sol;Nelson,JStuart
红外测量已被用于生物组织的轮廓或成像，包括人体皮肤。通常，对这类测量的分析假设红外吸收是由于水和胶原蛋白。这样的假设对于软组织可能是合理的，但是将外源性物质引入皮肤或测量组织幻象就提出了其红外吸收光谱的问题。我们使用衰减全反射模式的傅里叶变换红外光谱测量了水、聚丙烯酰胺、脂肪内酯、胶原凝胶、四种高渗清除剂（甘油、1,3 -丁二醇、三甲基丙烷、Topicare™）和离体人角质层和真皮在2-15µm范围内的红外吸收光谱。幻影材料的吸收光谱与水相似，尽管在6-10µm范围内发现了额外的结构。清净剂的吸收光谱较为复杂，在6 ~ 12µm之间以分子吸收带为主。真皮与水相似，在6-10µm范围内有明显的胶原结构。由于角质层含水量较低，其吸收量明显低于真皮层。这些结果表明，在2.5-6µm范围内以水为主的吸收假设是有效的。在较长波长的波段，清洗剂的吸收光谱与水的吸收光谱有很大的不同。该光谱信息可用于脉冲光热辐射测量，或用于使用常数μ ir的重建解释。在这种情况下，高估μ ir会低估发色团深度，反之亦然，尽管效果取决于实际的发色团深度。
Infrared measurements have been used to profile or image biological tissue, including human skin. Usually, analysis of such measurements has assumed that infrared absorption is due to water and collagen. Such an assumption may be reasonable for soft tissue, but introduction of exogenous agents into skin or the measurement of tissue phantoms has raised the question of their infrared absorption spectrum. We used Fourier transform infrared spectroscopy in attenuated total reflection mode to measure the infrared absorption spectra, in the range of 2–15 µm, of water, polyacrylamide, Intralipid, collagen gels, four hyperosmotic clearing agents (glycerol, 1, 3-butylene glycol, trimethylolpropane, Topicare™), and ex vivo human stratum corneum and dermis. The absorption spectra of the phantom materials were similar to that of water, although additional structure was noted in the range of 6–10 µm. The absorption spectra of the clearing agents were more complex, with molecular absorption bands dominating between 6 and 12 µm. Dermis was similar to water, with collagen structure evident in the 6–10 µm range. Stratum corneum had a significantly lower absorption than dermis due to a lower content of water. These results suggest that the assumption of water-dominated absorption in the 2.5–6 µm range is valid. At longer wavelengths, clearing agent absorption spectra differ significantly from the water spectrum. This spectral information can be used in pulsed photothermal radiometry or utilized in the interpretation of reconstructions in which a constant μ ir is used. In such cases, overestimating μ ir will underestimate chromophore depth and vice versa, although the effect is dependent on actual chromophore depth.
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丙烯酰胺和咔唑共电聚合膜的光学、热学和电化学性能
影响因子：
4.4
作者：
O. Yavuz;L. Berlouis;M. Hitchman;A. Sarac
通讯作者：
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使用脉冲光热辐射测量测量地下温度的局限性。
影响因子：
3.5
作者：
U. Sathyam;S. Prahl
通讯作者：
S. Prahl
影响因子：
2.4
作者：
FLOCK, ST;JACQUES, SL;VANGEMERT, MJC
通讯作者：
VANGEMERT, MJC
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影响因子：
4.4
作者：
A. Sarac;G. Sonmez;B. Ustamehmetoğlu
通讯作者：
B. Ustamehmetoğlu
DOI：
10.1364/josaa.12.001479
发表时间：
1995-07-01
影响因子：
1.9
作者：
MILNER, TE;GOODMAN, DM;NELSON, JS
通讯作者：
NELSON, JS