Accumulation of hyaluronan in bronchoalveolar lavage fluid is independent of iron-, complement- and granulocyte-depletion in bleomycin-induced alveolitis in the rat.
Accumulation of hyaluronan in bronchoalveolar lavage fluid is independent of iron-, complement- and granulocyte-depletion in bleomycin-induced alveolitis in the rat.
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在博莱霉素诱导的大鼠肺泡炎中，支气管肺泡灌洗液中透明质酸的积累与铁、补体和粒细胞的消耗无关。
DOI：
10.1183/09031936.93.03070765
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
R. Hällgren
中科院分区：
文献类型：
--
作者：
O. Nettelbladt;K. Lundberg;A. Tengblad;R. Hällgren
先前关于博来霉素诱导的大鼠肺泡炎的研究已经证明了透明质酸（透明质酸盐或透明质酸）在肺泡上皮中的短暂组织学蓄积，对应于支气管肺泡灌洗液（BAL）和肺组织提取物中透明质酸（HA）水平的增加。HA的蓄积与BALF中炎性细胞尤其是多形核细胞（PMNs）的内流及肺水的增加有关。在这项研究中，我们研究了铁，补体和中性粒细胞依赖性机制对肺早期连接反应的博莱霉素大鼠模型的影响。铁耗竭对HA或博莱霉素后第4天回收的灌洗液的细胞组成没有影响。用眼镜蛇毒因子（CVF）治疗博来霉素损伤的大鼠，完全中和了补体溶血活性，但对灌洗液HA或BAL中的细胞侵袭没有影响。用抗中性粒细胞血清（ANS）治疗，使血液和灌洗液PMN减少70- 80%，但对BAL中的HA水平没有影响。这些结果表明，除了依赖于铁、补体激活或PMN募集的调节机制之外，其他调节机制是导致该纤维化肺泡炎动物模型中HA蓄积的原因。
Previous studies on bleomycin-induced alveolitis in rats have demonstrated a transient histological accumulation of hyaluronan (hyaluronate or hyaluronic acid) in the alveolar interstitium, corresponding to increases in hyaluronan (HA) levels in bronchoalveolar lavage (BAL) fluid and lung tissue extracts. The accumulation of HA was related to the influx of inflammatory cells, especially polymorphonucleated cells (PMNs) in BAL fluid and the increase in lung water. In this study we have investigated the influence of iron, complement and PMN dependent mechanisms on the early connective response of the lung in the bleomycin rat model. Iron depletion had no effect on HA or the cellular composition of lavage fluid recovered on day 4 post bleomycin. Treatment of bleomycin-injured rats with cobra venom factor (CVF), totally neutralized complement haemolytic activity but had no effect on lavage HA or the cell invasion in BAL. Treatment with anti-neutrophil serum (ANS), reduced blood and lavage PMN by 70-80%, but had no influence on HA levels in BAL. These results suggest that regulatory mechanisms other than those dependent on iron, complement activation or PMN recruitment are responsible for HA accumulation in this fibrosing alveolitis animal model.
影响因子：
2
作者：
WEIGEL, PH;FULLER, GM;LEBOEUF, RD
通讯作者：
LEBOEUF, RD
博来霉素诱导仓鼠肺纤维化期间肺脯氨酰羟化酶和超氧化物歧化酶活性增加。
影响因子：
3.6
作者：
Giri,SN;Misra,HP;Chandler,DB;Chen,ZL
通讯作者：
Chen,ZL
影响因子：
9.6
作者：
PETERSON, MW;MONICK, M;HUNNINGHAKE, GW
通讯作者：
HUNNINGHAKE, GW