Light evokes rapid circadian network oscillator desynchrony followed by gradual phase retuning of synchrony.
Light evokes rapid circadian network oscillator desynchrony followed by gradual phase retuning of synchrony.
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DOI：
10.1016/j.cub.2015.01.056
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发表时间：
2015-03-30
期刊：
影响因子：
9.2
通讯作者：
Holmes, Todd C.
中科院分区：
文献类型：
--
作者：
Roberts, Logan;Leise, Tanya L.;Noguchi, Takako;Galschiodt, Alexis M.;Houl, Jerry H.;Welsh, David K.;Holmes, Todd C.
昼夜神经回路产生近24小时的生理节律，其可以被光夹带以协调动物生理与每日太阳周期。为了研究昼夜节律电路如何重组其活动，以响应光，我们成像周期（每）时钟基因循环长达6天，在单神经元分辨率的全脑外植体培养制备的荧光素酶转基因苍蝇。我们比较了培养物进行相位推进光脉冲（LP）的文化保持在黑暗中（DD）。在DD中，所有昼夜节律亚组中的单个神经元振荡器最初同步良好，然后显示振荡器节律振幅单调下降并与时间同步。s-LNvs和LNds以较慢的相对速率表现出这种降低。相比之下，LP引起的振荡器之间和大多数昼夜神经元亚群内的同步性的快速损失，然后逐渐相位重新调整整个电路振荡器同步性。LNds保持高节律幅度和同步性，跟随LP沿着最快速的相干相位提前。PER的免疫细胞化学分析表明，DD和LP中的这些动态在体内重现。解剖学上不同的昼夜神经元亚组在它们对LP的响应上不同，显示出它们的损失、恢复和/或加强同步性和节律性的程度和动力学的差异。短暂的昼夜节律似乎是果蝇多细胞生物钟光反应的一个重要特征。在不同的昼夜节律回路的神经元亚群中的个体振荡器显示出不同的光响应和相位重调的动力学特征。
Circadian neural circuits generate near 24 hr physiological rhythms that can be entrained by light to coordinate animal physiology with daily solar cycles. To examine how a circadian circuit reorganizes its activity in response to light, we imaged period (per) clock gene cycling for up to 6 days at single neuron resolution in whole brain explant cultures prepared from per-luciferase transgenic flies. We compared cultures subjected to a phase-advancing light pulse (LP) to cultures maintained in darkness (DD). In DD, individual neuronal oscillators in all circadian subgroups are initially well synchronized, then show monotonic decrease in oscillator rhythm amplitude and synchrony with time. The s-LNvs and LNds exhibit this decrease at a slower relative rate. In contrast, the LP evokes a rapid loss of oscillator synchrony between and within most circadian neuronal subgroups followed by gradual phase retuning of whole circuit oscillator synchrony. The LNds maintain high rhythmic amplitude and synchrony following the LP along with the most rapid coherent phase advance. Immunocytochemical analysis of PER show these dynamics in DD and LP are recapitulated in vivo. Anatomically distinct circadian neuronal subgroups vary in their response to the LP, showing differences in the degree and kinetics of their loss, recovery and/or strengthening of synchrony and rhythmicity. Transient desynchrony appears to be an integral feature of light response of the Drosophila multicellular circadian clock. Individual oscillators in different neuronal subgroups of the circadian circuit show distinct kinetic signatures of light response and phase retuning.
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影响因子：
16.2
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Evans JA;Leise TL;Castanon-Cervantes O;Davidson AJ
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