In Vivo Display of a Multisubunit Enzyme Complex on Biogenic Magnetic Nanoparticles
In Vivo Display of a Multisubunit Enzyme Complex on Biogenic Magnetic Nanoparticles
复制标题
生物磁性纳米颗粒上多亚基酶复合物的体内展示
DOI：
10.1128/aem.01640-09
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发表时间：
2009
影响因子：
4.4
通讯作者：
D. Schüler
中科院分区：
文献类型：
--
作者：
S. Ohuchi;D. Schüler
摘要磁小体是由趋磁细菌产生的具有磁性晶体的细菌细胞器。磁小体具有良好的物理化学性质，是人工展示高度复杂生物复合物的理想支架。作为一个模型实验磁小体上的多亚基复合物的功能表达，我们研究了显示的嵌合细菌RNase P酶组成的蛋白质亚基（C5）的大肠杆菌RNase P和内源性RNA亚基通过表达C5与MamC，一种已知的磁小体蛋白，在趋磁细菌Magnetoelillum gryphiswaldense翻译融合。正如预期的，纯化的C5融合磁小体，而不是野生型磁小体，显示出明显的RNase P活性和典型的细菌RNase P RNA的关联。我们的结果首次证明磁小体可以用作展示多亚基复合物的支架。
ABSTRACT Magnetosomes are unique bacterial organelles comprising membrane-enveloped magnetic crystals produced by magnetotactic bacteria. Because of several desirable chemical and physical properties, magnetosomes would be ideal scaffolds on which to display highly complicated biological complexes artificially. As a model experiment for the functional expression of a multisubunit complex on magnetosomes, we examined the display of a chimeric bacterial RNase P enzyme composed of the protein subunit (C5) of Escherichia coli RNase P and the endogenous RNA subunit by expressing a translational fusion of C5 with MamC, a known magnetosome protein, in the magnetotactic bacterium Magnetospirillum gryphiswaldense. As intended, the purified C5 fusion magnetosomes, but not wild-type magnetosomes, showed apparent RNase P activity and the association of a typical bacterial RNase P RNA. Our results demonstrate for the first time that magnetosomes can be employed as scaffolds for the display of multisubunit complexes.
DOI：
10.1073/pnas.85.4.1033
发表时间：
1988-02-01
影响因子：
11.1
作者：
SAMPSON, JR;UHLENBECK, OC
通讯作者：
UHLENBECK, OC
影响因子：
4.5
作者：
Harris, JK;Haas, ES;Brown, JW
通讯作者：
Brown, JW