Differential Regulation of Adhesion and Phagocytosis of Resting and Activated Microglia by Dopamine.
Differential Regulation of Adhesion and Phagocytosis of Resting and Activated Microglia by Dopamine.
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DOI：
10.3389/fncel.2018.00309
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发表时间：
2018
影响因子：
5.3
通讯作者：
Chung CY
中科院分区：
文献类型：
--
作者：
Fan Y;Chen Z;Pathak JL;Carneiro AMD;Chung CY
Microglia, the immune competent cells of the central nervous system (CNS), normally exist in a resting state characterized by a ramified morphology with many processes, and become activated to amoeboid morphology in response to brain injury, infection, and a variety of neuroinflammatory stimuli. Many studies focused on how neurotransmitters affect microglia activation in pathophysiological circumstances. In this study, we tried to gain mechanistic insights on how dopamine (DA) released from neurons modulates cellular functions of resting and activated microglia. DA induced the reduction of the number of cellular processes, the increase of cell adhesion/spreading, and the increase of vimentin filaments in resting primary and BV2 microglia. In contrast to resting cells, DA downregulated the cell spreading and phagocytosis of microglia activated by LPS. DA also significantly downregulated ERK1/2 phosphorylation in activated microglia, but not in resting microglia. Downregulation of ERK1/2 by DA in activated microglia required receptor signaling. In contrast, we found a significant increase of p38MAPK activity by DA treatment in resting, but not in activated microglia. These latter effects required the uptake of DA through the high-affinity transporter but did not require receptor signaling. Activation of p38MAPK resulted in the increase of focal adhesion number via phosphorylation of paxillin at Ser83. These results indicate that DA might have a differential, depending upon the activation stage of microglia, impact on cellular functions such as adhesion and phagocytosis.
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影响因子：
3.8
作者：
Fan Y;Xie L;Chung CY
通讯作者：
Chung CY
影响因子：
5.3
作者：
Gitik M;Kleinhaus R;Hadas S;Reichert F;Rotshenker S
通讯作者：
Rotshenker S
影响因子：
3.7
作者：
Jeong YH;Kim Y;Song H;Chung YS;Park SB;Kim HS
通讯作者：
Kim HS
p38mapk对帕西林的磷酸化参与PC-12细胞的神经突延伸。
DOI：
10.1083/jcb.200307081
发表时间：
2004-02-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Huang C;Borchers CH;Schaller MD;Jacobson K
通讯作者：
Jacobson K
影响因子：
15.1
作者：
Krabbe, Grietje;Matyash, Vitali;Kettenmann, Helmut
通讯作者：
Kettenmann, Helmut